Recombinant expression and purification of phenylalanyl-tRNA synthetase from wheat: a long-lasting poly(U)-dependent poly(Phe) synthesis system,Preparative Biochemistry & Biotechnology

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Recombinant expression and purification of phenylalanyl-tRNA synthetase from wheat: a long-lasting poly(U)-dependent poly(Phe) synthesis system
Preparative Biochemistry & Biotechnology ( IF 2.9 ) Pub Date : 2024-03-05 , DOI: 10.1080/10826068.2024.2324077
Haruyuki Furukawa ₁ , Yuto Nagashio ₁ , Kensuke Tsutsumi ₁ , Naofumi Matsubara ₁ , Ryohei Kato ₁ , Chie Tomikawa ₁ , Kazuyuki Takai ₁

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Synthetic genes for the two subunits of phenylalanyl-tRNA synthetase (PheRS) from wheat were expressed in Escherichia coli. When each gene was induced individually, the α subunit with a cleavable 6...

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