Islet or β-cell transplantation is currently considered to be the ideal treatment for diabetes, and three-dimensional (3D) bioprinting of a bionic pancreas with physiological stiffness is considered to be promising for the encapsulation and transplantation of β-cells. In this study, a 5%GelMA/2%AlgMA hybrid hydrogel with pancreatic physiological stiffness was constructed and used for β-cell encapsulation, 3D bioprinting, and in vivo transplantation to evaluate glycemic control in diabetic mice. The hybrid hydrogel had good cytocompatibility and could induce insulin-producing cells (IPCs) to form pseudoislet structures and improve insulin secretion. Furthermore, we validated the importance of betacellulin (BTC) in IPCs differentiation and confirmed that IPCs self-regulation was achieved by altering the nuclear and cytoplasmic distributions of BTC expression. In vivo transplantation of diabetic mice quickly restored blood glucose levels. In the future, 3D bioprinting of β-cells using biomimetic hydrogels will provide a promising platform for clinical islet transplantation for the treatment of diabetes.

中文翻译：

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仿生水凝胶促进伪胰岛形成，改善糖尿病小鼠的血糖控制

胰岛或β细胞移植目前被认为是糖尿病的理想治疗方法，而具有生理硬度的仿生胰腺的三维（3D）生物打印被认为对于β细胞的封装和移植有希望。在本研究中，构建了具有胰腺生理硬度的 5%GelMA/2%AlgMA 混合水凝胶，并将其用于 β 细胞封装、3D 生物打印和体内移植，以评估糖尿病小鼠的血糖控制。杂化水凝胶具有良好的细胞相容性，可以诱导胰岛素生成细胞（IPC）形成假胰岛结构并改善胰岛素分泌。此外，我们验证了β细胞素（BTC）在IPC分化中的重要性，并证实IPC的自我调节是通过改变BTC表达的核和细胞质分布来实现的。糖尿病小鼠的体内移植很快恢复了血糖水平。未来，使用仿生水凝胶对β细胞进行3D生物打印将为临床胰岛移植治疗糖尿病提供一个有前途的平台。