Long non-coding RNAs (lncRNAs) appear to be the crucial modulators in various processes and critically influence the oncogenesis. As one of the LncRNAs, LncRNA CCAT1 has been reported to be closely associated with the progression multiple cancers, but its role in modulating the radioresistance of lung adenocarcinoma (LUAD) remains unclear. In our present study, we screened the potential radioresistance related LncRNAs in LUAD based on the data from The Cancer Genome Atlas (TCGA) database. Data suggested that CCAT1 was abundantly expressed in LUAD and CCAT1 was significantly associated with poor prognosis and radioresistance. Moreover, our in vitro experiments showed that radiation treatment could trigger elevated expression of CCAT1 in the human LUAD cell lines. Further loss/gain-of-function investigations indicated that CCAT1 knockdown significantly inhibited cell proliferation, migration and promoted cell apoptosis in NCI-H1299 cells under irradiation, whereas CCAT1 overexpression in A549 cells yield the opposite effects. In summary, we identified the promoting role of CCAT1 in radioresistance of LUAD, which may provide a theoretical basis for radiotherapy sensitization of LUAD.

长链非编码 RNA (lncRNA) 似乎是各种过程中的关键调节剂，并对肿瘤发生产生重要影响。作为LncRNA之一，LncRNA CCAT1已被报道与多种癌症的进展密切相关，但其在调节肺腺癌（LUAD）放射抗性中的作用仍不清楚。在我们目前的研究中，我们根据癌症基因组图谱（TCGA）数据库的数据筛选了 LUAD 中潜在的放射抗性相关的 LncRNA。数据表明，CCAT1 在 LUAD 中大量表达，并且 CCAT1 与不良预后和放射抗性显着相关。此外，我们的体外实验表明，放射治疗可以触发人类 LUAD 细胞系中 CCAT1 的表达升高。进一步的功能丧失/获得研究表明，在辐射下，CCAT1 敲低显着抑制 NCI-H1299 细胞的细胞增殖、迁移并促进细胞凋亡，而 A549 细胞中 CCAT1 过表达则产生相反的效果。综上所述，我们确定了CCAT1在LUAD放射抵抗中的促进作用，这可能为LUAD放射治疗增敏提供理论依据。