The spillage of petroleum hydrocarbons, one of the most versatile energy resources, leads to disastrous environmental pollution. The present study aims to degrade oil using enzymes from bacterial strains. A total of 39 bacteria were isolated from six different soil samples collected from Ullal Beach, Mangalore, Karnataka, located at 12°52′N latitude and 74°49′E longitude, India. All 39 bacterial isolates were screened for the production of four industrially important extracellular enzymes. Among these isolates, ten showed the highest lipase production. These cultures were further screened for bio-surfactant assays, including oil displacement and drop collapse assay and Emulsification Index. EBPL0613-F2 exhibited the best reaction in crude oil degradation. A polyphasic taxonomical approach identified the crude oil-degrading bacterium EBPL0613-F2 as Bacillus licheniformis and submitted in NCBI and the Accession Number is PP059616. It was then cultivated in ocean water media with tween 20 and 1% crude oil as the sole carbon and energy source. The strain was screened for lipase quantitative and qualitative assay and the protein content was also estimated. The identified bacterial strain Bacillus licheniformis EBPL0613-F2 demonstrated moderate lipase activity, with 76 U/ml and 24 U/ml, respectively, after 48–72 h of incubation in the crude oil substrate. For Tween 20 substrates, it exhibited 36 U/ml and 34 U/ml, respectively. FTIR analysis was used to examine the properties of crude oil following the biodegradation. The results suggest that, EBPL0613-F2 recorded the highest degradation rate so this culture has the potential for use in the degradation of crude oil in a greener manner.

石油碳氢化合物是用途最广泛的能源之一，其泄漏会导致灾难性的环境污染。本研究旨在利用细菌菌株中的酶来降解油。从位于印度北纬 12°52′、东经 74°49′卡纳塔克邦门格洛尔乌拉尔海滩采集的 6 个不同土壤样品中分离出总共 39 种细菌。筛选了所有 39 种细菌分离株，以生产四种工业上重要的胞外酶。在这些分离株中，有十种显示出最高的脂肪酶产量。对这些培养物进行了生物表面活性剂测定的进一步筛选，包括驱油和滴塌陷测定以及乳化指数。EBPL0613-F2在原油降解中表现出最好的反应。多相分类学方法将原油降解细菌 EBPL0613-F2 鉴定为地衣芽孢杆菌，并提交到 NCBI，登录号为 PP059616。然后将其培养在海水介质中，以吐温 20 和 1% 原油作为唯一的碳和能源。对菌株进行脂肪酶定量和定性测定筛选，并估计蛋白质含量。鉴定出的细菌菌株地衣芽孢杆菌EBPL0613-F2 在原油底物中孵育 48-72 小时后表现出中等的脂肪酶活性，分别为 76 U/ml 和 24 U/ml。对于 Tween 20 底物，其分别表现出 36 U/ml 和 34 U/ml。FTIR 分析用于检查生物降解后原油的特性。结果表明，EBPL0613-F2 记录了最高的降解率，因此该培养物具有以更绿色的方式用于原油降解的潜力。