Gravitational changes between micro- and hypergravity cause several adaptations and alterations in the human body. Besides muscular atrophy and immune system impairment, effects on the circulatory system have been described, which can be associated with a wide range of blood biomarker changes. This study examined nine individuals (seven males, two females) during a parabolic flight campaign (PFC). Thirty-one parabolas were performed in one flight day, resulting in ~22 s of microgravity during each parabola. Each participant was subjected to a single flight day with a total of 31 parabolas, totaling 11 min of microgravity during one parabolic flight. Before and after (1 hour (h) and 24 h), the flights blood was sampled to examine potential gravity-induced changes of circulating plasma proteins. Proximity Extension Assay (PEA) offers a proteomic solution, enabling the simultaneous analysis of a wide variety of plasma proteins. From 2925 unique proteins analyzed, 251 (8.58%) proteins demonstrated a differential regulation between baseline, 1 h and 24 h post flight. Pathway analysis indicated that parabolic flights led to altered levels of proteins associated with vesicle organization and apoptosis up to 24 h post microgravity exposure. Varying gravity conditions are associated with poorly understood physiological changes, including stress responses and fluid shifts. We provide a publicly available library of gravity-modulated circulating protein levels illustrating numerous changes in cellular pathways relevant for inter-organ function and communication.

微重力和超重力之间的重力变化会导致人体发生一些适应和改变。除了肌肉萎缩和免疫系统损伤之外，还描述了对循环系统的影响，这可能与广泛的血液生物标志物变化有关。这项研究在抛物线飞行运动 (PFC) 期间检查了九个人（七名男性，两名女性）。一个飞行日执行了 31 次抛物线飞行，每次抛物线飞行期间产生约 22 秒的微重力。每个参与者在一天的飞行中总共经历了 31 条抛物线，一次抛物线飞行期间总计 11 分钟的微重力。之前和之后（1 小时 (h) 和 24 小时），对航班进行血液采样，以检查循环血浆蛋白可能由重力引起的变化。邻近延伸分析 (PEA) 提供了一种蛋白质组解决方案，能够同时分析多种血浆蛋白。在分析的 2925 种独特蛋白质中，有 251 种 (8.58%) 蛋白质表现出基线、飞行后 1 小时和 24 小时之间的差异调节。通路分析表明，在微重力暴露后长达 24 小时内，抛物线飞行会导致与囊泡组织和细胞凋亡相关的蛋白质水平发生变化。不同的重力条件与人们知之甚少的生理变化有关，包括压力反应和体液变化。我们提供了一个公开的重力调节循环蛋白水平库，说明了与器官间功能和通讯相关的细胞途径的众多变化。