Androgenic alopecia (AGA) is a highly prevalent form of non-scarring alopecia but lacks effective treatments. Stem cell exosomes have similar repair effects to stem cells, suffer from the drawbacks of high cost and low yield yet. Cell-derived nanovesicles acquired through mechanical extrusion exhibit favorable biomimetic properties similar to exosomes, enabling them to efficiently encapsulate substantial quantities of therapeutic proteins. In this study, we observed that JAM-A, an adhesion protein, resulted in a significantly increased the adhesion and resilience of dermal papilla cells to form snap structures against damage caused by dihydrotestosterone and macrophages, thereby facilitating the process of hair regrowth in cases of AGA. Consequently, adipose-derived stem cells were modified to overexpress JAM-A to produce engineered JAM-A overexpressing nanovesicles (JAM-A@NV). The incorporation of JAM-A@NV into a thermosensitive hydrogel matrix (JAM-A@NV Gel) to effectively addresses the limitations associated with the short half-life of JAM-A@NV, and resulted in the achievement of a sustained-release profile for JAM-A@NV. The physicochemical characteristics of the JAM-A@NV Gel were analyzed and assessed for its efficacy in promoting hair regrowth in vivo and vitro. The JAM-A@NV Gel, thus, presents a novel therapeutic approach and theoretical framework for promoting the treatment of low cell adhesion diseases similar to AGA.

中文翻译：

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仿生工程ADSC纳米囊泡热敏水凝胶增强真皮乳头细胞自噬用于雄激素性脱发治疗

雄激素性脱发（AGA）是一种非常普遍的非疤痕性脱发，但缺乏有效的治疗方法。干细胞外泌体具有与干细胞类似的修复作用，但存在成本高、产量低的缺点。通过机械挤压获得的细胞源性纳米囊泡表现出与外泌体相似的良好仿生特性，使它们能够有效地封装大量的治疗性蛋白质。在这项研究中，我们观察到 JAM-A（一种粘附蛋白）可显着增加真皮乳头细胞的粘附力和弹性，形成弹性结构，抵抗二氢睾酮和巨噬细胞造成的损伤，从而促进毛发再生过程。阿嘎。因此，脂肪干细胞被修饰以过度表达 JAM-A，以产生工程化的 JAM-A 过度表达纳米囊泡 (JAM-A@NV)。将 JAM-A@NV 纳入热敏水凝胶基质（JAM-A@NV Gel）中，有效解决了 JAM-A@NV 半衰期短的限制，并实现了缓释JAM-A@NV 的配置文件。对 JAM-A@NV 凝胶的理化特性进行了分析和评估，以了解其在体内和体外促进毛发再生的功效。因此，JAM-A@NV Gel 提出了一种新的治疗方法和理论框架，用于促进与 AGA 类似的低细胞粘附疾病的治疗。