The biological responses of MTA and Biodentine™ has been assessed on a three-dimensional, tissue-engineered organotypic deciduous pulp analogue. Human endothelial (HUVEC) and dental mesenchymal stem cells (SHED) at a ratio of 3:1, were incorporated into a collagen I/fibrin hydrogel; succeeding Biodentine™ and MTA cylindrical specimens were placed in direct contact with the pulp analogue 48 h later. Cell viability/proliferation and morphology were evaluated through live/dead staining, MTT assay and Scanning Electron Microscopy (SEM), and expression of angiogenic, odontogenic markers through real time PCR. Viable cells dominated at day 3 after treatment presenting typical morphology, firmly attached within the hydrogel structures, as shown by live/dead staining and SEM images. MTT assay at day 1 presented a significant increase of cell proliferation in Biodentine™ group. Real-time PCR showed significant upregulation of odontogenic markers , (day 3,6), , (day 3) in contact with Biodentine™ compared to MTA and the control, whereas MTA promoted significant upregulation of , , , (day 3) and (day 6) compared to the control. presented downregulation in both experimental groups. Expression of angiogenic markers and at day 3 was significantly upregulated in contact with Biodentine™ and MTA respectively, while the receptors , and , as well as were downregulated. Both calcium silicate-based materials are biocompatible and exert positive angiogenic and odontogenic effects, although Biodentine™ during the first days of culture, seems to induce higher cell proliferation and provoke a more profound odontogenic and angiogenic response from SHED.

中文翻译：

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“两种硅酸钙基水泥对组织工程 3D 器官型乳牙髓类似物的生物反应”

MTA 和 Biodentine™ 的生物反应已在三维组织工程器官型乳牙髓类似物上进行了评估。将人内皮细胞 (HUVEC) 和牙科间充质干细胞 (SHED) 以 3:1 的比例掺入 I 型胶原蛋白/纤维蛋白水凝胶中；48 小时后，将后续的 Biodentine™ 和 MTA 圆柱形标本与牙髓类似物直接接触。通过活/死染色、MTT 测定和扫描电子显微镜 (SEM) 评估细胞活力/增殖和形态，并通过实时 PCR 评估血管生成、牙源标记物的表达。处理后第 3 天，活细胞占主导地位，呈现出典型的形态，牢固地附着在水凝胶结构内，如活/死染色和 SEM 图像所示。第 1 天的 MTT 检测显示 Biodentine™ 组的细胞增殖显着增加。实时 PCR 显示，与 MTA 和对照相比，与 Biodentine™ 接触的牙源标记物 , （第 3,6 天）, , （第 3 天）显着上调，而 MTA 促进 , , , （第 3 天）和（第 6 天）与对照相比。两个实验组均呈现下调。在与 Biodentine™ 和 MTA 接触的第 3 天，血管生成标记物 和 的表达分别显着上调，而受体 、 和 、 以及 则下调。两种基于硅酸钙的材料都具有生物相容性，并发挥积极的血管生成和成牙作用，尽管 Biodentine™ 在培养的第一天似乎会诱导更高的细胞增殖，并激发 SHED 更深刻的成牙和血管生成反应。