Abstract—Breast cancer is the most common type of cancer among women. The study of the mechanisms of metastasis, the main cause of death from breast cancer, as well as the search for new markers for early diagnosis and prognosis of breast cancer, is an extremely topical issue. New perspectives in the diagnosis and treatment of breast cancer are opened by the mechanisms of gene regulation involving non-coding RNAs, in particular, long non-coding RNAs (lncRNAs). In this work, we analyzed the methylation levels of seven lncRNA genes (MEG3, SEMA3B-AS1, HAND2-AS1, KCNK15-AS1, ZNF667-AS1, MAGI2-AS3, and PLUT) by quantitative methyl-specific PCR on a set of 79 paired (tumor/normal) samples of breast cancer. Hypermethylation of all seven lncRNA genes was revealed, and hypermethylation of HAND2-AS1, KCNK15-AS1, MAGI2-AS3, and PLUT was detected in breast cancer for the first time. It was found that the level of methylation of the studied lncRNA genes correlated statistically significantly with the stage of the tumor process, the size of the tumor, and the presence of metastases in the lymph nodes. Thus, methylation of the seven studied lncRNA genes is associated with the development and progression of breast cancer, and these genes can be useful as potential markers in the diagnosis and prognosis of breast cancer.

摘要：乳腺癌是女性中最常见的癌症类型。作为乳腺癌主要死亡原因的转移机制的研究，以及寻找乳腺癌早期诊断和预后的新标志物，是一个非常热门的话题。涉及非编码RNA，特别是长链非编码RNA（lncRNA）的基因调控机制为乳腺癌的诊断和治疗开辟了新的视角。在这项工作中，我们通过定量甲基特异性 PCR 对一组 79个 lncRNA 基因（ MEG3、SEMA3B-AS1、HAND2-AS1、KCNK15-AS1、ZNF667-AS1、MAGI2-AS3和PLUT ）进行了甲基化水平分析。乳腺癌的配对（肿瘤/正常）样本。揭示了所有七个lncRNA基因的高甲基化，并且首次在乳腺癌中检测到HAND2-AS1、KCNK15-AS1、MAGI2-AS3和PLUT的高甲基化。研究发现，所研究的lncRNA基因的甲基化水平与肿瘤进展阶段、肿瘤大小以及淋巴结转移的存在具有统计学显着相关性。因此，所研究的7个lncRNA基因的甲基化与乳腺癌的发生和进展相关，这些基因可作为乳腺癌诊断和预后的潜在标志物。