Abstract—Regulation of retrotransposon activity in somatic tissues is a complex mechanism that has still not been studied in detail. It is strongly believed that siRNA interference is main mechanism of retrotransposon activity regulation outside the gonads, but recently was demonstrated that piRNA interference participates in retrotransposon repression during somatic tissue development. In this work, using RT-PCR, we demonstrated that during ontogenesis piRNA interference determinates retrotransposon expression level on imago stage and retrotransposons demonstrate tissue-specific expression. The major factor of retrotransposon tissue-specific expression is presence of transcription factor binding sites in their regulatory regions.

摘要：体细胞组织中逆转录转座子活性的调节是一个复杂的机制，尚未得到详细研究。人们强烈认为siRNA干扰是性腺外逆转录转座子活性调节的主要机制，但最近证明piRNA干扰参与体细胞组织发育过程中逆转录转座子的抑制。在这项工作中，我们使用 RT-PCR 证明，在个体发生过程中，piRNA 干扰决定了成虫期逆转录转座子的表达水平，并且逆转录转座子表现出组织特异性表达。逆转录转座子组织特异性表达的主要因素是其调节区域中转录因子结合位点的存在。