In the present study, the effects of 60Co-γ irradiation on the diversity of fungal microflora in blueberries during cold storage have been explored in detail. The effects of irradiation on the fungal microflora in blueberries could be observed at the lowest effective dose of 1.0 kGy. The genome library of fungal microflora in blueberries was evaluated by tusing Shannon, Simpson, Chao1, and ACE indices, presenting good coverage of blueberry samples. Principal coordinate analysis clarified the differences in the evolution and clustering of fungi in blueberries under different irradiation levels. The composition and diversity of fungal microflora in blueberries treated with different doses of radiation exhibited significant differences under cold storage of 4°C. However, most of the fungi belonged to the Ascomycota at the phylum. The fungal microflora was relatively stable at the initial stage of cold storage, however, the diversity of fungal species in irradiated blueberries varied greatly in the middle stage around the 15th day. This comprehensive study provides deeper insight about the effects of irradiation on the evolution of fungal microflora in blueberries during cold storage. The findings of this study lay a good foundation for post-harvest storage and preservation of blueberries.

中文翻译：

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60Co γ辐照处理对蓝莓冷藏期间真菌菌群多样性的影响

本研究详细探讨了 60Co-γ 辐照对蓝莓冷藏期间真菌微生物多样性的影响。在最低有效剂量 1.0 kGy 下即可观察到辐照对蓝莓真菌微生物区系的影响。通过使用Shannon、Simpson、Chao1和ACE指数对蓝莓真菌微生物区系基因组文库进行评估，显示了蓝莓样品的良好覆盖率。主坐标分析阐明了不同辐照水平下蓝莓真菌进化和聚类的差异。4℃冷藏条件下，不同剂量辐射处理的蓝莓真菌菌群组成和多样性表现出显着差异。然而，大多数真菌属于子囊菌门。冷藏初期真菌菌群相对稳定，但在第15天左右的中期，辐照蓝莓的真菌种类多样性变化较大。这项综合研究提供了关于辐照对冷藏期间蓝莓真菌微生物群落进化的影响的更深入的见解。本研究结果为蓝莓采后贮藏保鲜奠定了良好的基础。