GPAT (sn-Glycerol-3-phosphate-1-O-acyltransferase) is a key enzyme in the biosynthesis of membrane lipids. Two kinds of GPATs are involved in the production of glycerolipids. While sn-1-GPATs are responsible for creating lysophosphatidic acid, sn-2-GPATs generate monoacylglycerol. GPAT genes of beans (Phaseolus vulgaris) have never been investigated before. This study aimed to assess the expression profiles of GPAT genes in common bean leaves under drought and salt stress and apply bioinformatics tools to the genome-wide analysis of GPAT gene family members. In the P. vulgaris genome, PvGPAT genes with molecular weights ranging from 50.2 to 60.4 kDa, amino acid counts ranging from 376 to 539, theoretical isoelectric points ranging from 8.41 to 9.46, and instability indices ranging from 31.84 to 51.38 were found. Based on the gene structure analysis, the expected number of exons and introns was between 2 and 12. The GPAT proteins from Arabidopsis thaliana, Glycine max, Vitis vinifera, Cicer arietinum, Lens culinaris, and P. vulgaris were analyzed phylogenetically. GPAT proteins of G. max and A. thaliana are evolutionarily related to those of beans. The PvGPAT4/PvGPAT10 and PvGPAT7/PvGPAT12 gene pairs were detected to be segmentally duplicated. In this work, qRT-PCR was performed on six PvGPAT genes, and the effects of salt, drought, and melatonin treatments on bean GPAT genes were studied. The role of melatonin in the response to salt and drought stress was investigated at the gene expression level. The findings of the present study will shed light on GPAT gene family members in beans and provide insight for future functional research.

GPAT（sn-甘油-3-磷酸-1-O-酰基转移酶）是膜脂生物合成中的关键酶。有两种GPAT参与甘油脂的生产。sn-1- GPAT负责产生溶血磷脂酸，而 sn-2- GPAT则产生单酰基甘油。豆类（Phaseolus vulgaris ）的GPAT基因以前从未被研究过。本研究旨在评估干旱和盐胁迫下菜豆叶片中GPAT基因的表达谱，并将生物信息学工具应用于GPAT基因家族成员的全基因组分析。在普通P. vulgaris基因组中，发现PvGPAT基因的分子量为 50.2 至 60.4 kDa，氨基酸数为 376 至 539，理论等电点为 8.41 至 9.46，不稳定指数为 31.84 至 51.38。根据基因结构分析，预计外显子和内含子的数量在 2 至 12 个之间。对来自拟南芥、大豆、葡萄、Cicer arietinum、Lens culinaris和P. vulgaris的 GPAT 蛋白进行系统发育分析。G. max和拟南芥的 GPAT 蛋白在进化上与豆类的蛋白相关。检测到PvGPAT4 /PvGPAT10和PvGPAT7/PvGPAT12基因对是分段重复的。在这项工作中，对六个PvGPAT基因进行了 qRT-PCR ，研究了盐、干旱和褪黑激素处理对豆类GPAT基因的影响。在基因表达水平上研究了褪黑激素在盐和干旱胁迫响应中的作用。本研究的结果将揭示豆类中的GPAT基因家族成员，并为未来的功能研究提供见解。