The present study outlines an easy, cheap, and environmentally friendly way to make Staphylococcus aureus-mediated bimetallic silver-copper nanocomposites (Ag/Cu) that can fight cancer and germs. The gram-positive S. aureus synthesized Ag, Cu, and their bi-metallic nanocomposites extracellularly. We aimed to prepare the bimetallic nanocomposite in two different ways, and we compared them in terms of characterization and biological applications. The first one is a bimetallic nanocomposite (Ag/Cu_b) that was made by mixing Ag and Cu metal ions in equal amounts (50:50). Then, the whole mixture was reduced. The second is the after-reduction bimetallic nanocomposite (Ag/Cu_a), in which each metal ion was reduced separately, and then the nanocomposites were mixed (50:50%) during biological applications. Nanocomposites were characterized using UV–visible spectrophotometry, Fourier-transform infrared spectroscopy, dynamic light scattering, and transmission electron microscopy. The results demonstrated that surface plasmon bands were at 320 nm for Ag NPs and 525 nm for Cu NPs, and a shift from these peaks was observed at 290 nm in the Ag/Cu_b bimetallic nanocomposite. The synthesized nanocomposites were confirmed to be in the nanoscale with 20, 40, and 80 nm spherical crystals, respectively. Nanocomposites were assayed for their antimicrobial activity against the gram-negative Pseudomonas aeruginosa, the acid-fast Mycobacterium smegmatis, the gram-positive Bacillus cereus, and S. aureus, in addition to three fungal species, which were Aspergillus flavus, A. fumigatus, and Candida albicans. The minimum inhibitory concentration and minimum bactericidal concentration were determined. The Ag/Cua/Cuaetallic nanocomposite was the most potent antimicrobial compound. The anticancer activity of the tested compounds was assayed against the hepatocellular carcinoma cell line (HepG-2). Low cytotoxic activity was recorded in most assayed nanocomposites against the baby hamster kidney cell line (BHK).

本研究概述了一种简单、廉价且环保的方法来制造金黄色葡萄球菌介导的双金属银铜纳米复合材料（Ag/Cu），该复合材料可以对抗癌症和细菌。革兰氏阳性金黄色葡萄球菌在细胞外合成银、铜及其双金属纳米复合材料。我们旨在以两种不同的方式制备双金属纳米复合材料，并在表征和生物应用方面对它们进行比较。第一种是双金属纳米复合材料 (Ag/Cu _b )，是通过等量 (50:50) 混合 Ag 和 Cu 金属离子制成的。然后，将整个混合物还原。第二种是还原后双金属纳米复合材料（Ag/Cu _a），其中每种金属离子分别被还原，然后在生物应用中将纳米复合材料混合（50:50%）。使用紫外可见分光光度法、傅里叶变换红外光谱法、动态光散射和透射电子显微镜对纳米复合材料进行了表征。_{结果表明，Ag NP 的表面等离子体激元带位于 320 nm，Cu NP 的表面等离子体激元带位于 525 nm，并且在 Ag/Cu b}双金属纳米复合材料中在 290 nm 处观察到这些峰的偏移。合成的纳米复合材料被证实为纳米级的，分别具有20、40和80 nm的球形晶体。测试了纳米复合材料对革兰氏阴性 铜绿假单胞菌、抗酸耻垢 分枝杆菌、革兰氏阳性蜡样 芽孢杆菌 和 金黄色葡萄球菌的抗菌活性， 以及​​三种真菌物种，即 黄曲霉、 烟曲霉、 和 白色念珠菌。测定最低抑菌浓度和最低杀菌浓度。Ag/Cua/Cuaetallic 纳米复合材料是最有效的抗菌化合物。测试化合物对肝细胞癌细胞系（HepG-2）的抗癌活性。大多数检测的纳米复合材料对幼仓鼠肾细胞系（BHK）的细胞毒性活性较低。