Abstract

Accidental events or surgical procedures usually lead to tissue injury. Fibrin sealants have proven to optimize the healing process but have some drawbacks due to their allogeneic nature. Autologous fibrin sealants present several advantages. The aim of this study is to evaluate the performance of a new autologous fibrin sealant based on Endoret®PRGF® technology (E-sealant). One of the most widely used commercial fibrin sealants (Tisseel®) was included as comparative Control. E-sealant´s hematological and biological properties were characterized. The coagulation kinetics and the microstructure were compared. Their rheological profile and biomechanical behavior were also recorded. Finally, the swelling/shrinkage capacity and the enzymatic degradation of adhesives were determined. E-sealant presented a moderate platelet concentration and physiological levels of fibrinogen and thrombin. It clotted 30 s after activation. The microstructure of E-sealant showed a homogeneous fibrillar scaffold with numerous and scattered platelet aggregates. In contrast, Control presented absence of blood cells and amorphous protein deposits. Although in different order of magnitude, both adhesives had similar rheological profiles and viscoelasticity. Control showed a higher hardness but both adhesives presented a pseudoplastic hydrogel nature with a shear thinning behavior. Regarding their adhesiveness, E-sealant presented a higher tensile strength before cohesive failure but their elastic stretching capacity and maximum elongation was similar. While E-sealant presented a significant shrinkage process, Control showed a slight swelling over time. In addition, E-sealant presented a high enzymatic resorption rate, while Control showed to withstand the biodegradation process in a significant way. E-sealant presents optimal biochemical and biomechanical properties suitable for its use as a fibrin sealant with regenerative purposes.

Graphical Abstract

中文翻译：

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用于再生医学的自体蛋白质纤维蛋白密封剂的生化和生物力学表征

摘要

意外事件或外科手术通常会导致组织损伤。纤维蛋白密封剂已被证明可以优化愈合过程，但由于其同种异体性质而存在一些缺点。自体纤维蛋白密封剂具有多种优点。本研究的目的是评估基于 Endoret®PRGF® 技术（E-密封剂）的新型自体纤维蛋白密封剂的性能。其中一种最广泛使用的商业纤维蛋白密封剂 (Tisseel®) 被纳入作为对比对照。对电子密封剂的血液学和生物学特性进行了表征。比较了凝固动力学和微观结构。还记录了它们的流变特性和生物力学行为。最后，测定了粘合剂的溶胀/收缩能力和酶降解能力。E-密封剂具有中等血小板浓度以及纤维蛋白原和凝血酶的生理水平。激活后 30 秒凝固。E-密封剂的微观结构显示出均匀的纤维状支架，具有大量分散的血小板聚集体。相反，对照表现出不存在血细胞和无定形蛋白沉积物。尽管数量级不同，但这两种粘合剂具有相似的流变特性和粘弹性。对照显示出较高的硬度，但两种粘合剂均呈现出具有剪切稀化行为的假塑性水凝胶性质。就其粘合性而言，电子密封胶在内聚破坏前表现出较高的拉伸强度，但其弹性拉伸能力和最大伸长率相似。虽然电子密封剂呈现出显着的收缩过程，但对照品随着时间的推移表现出轻微的膨胀。此外，E-密封剂呈现出高酶促吸收率，而对照显示出能够显着地承受生物降解过程。E-密封剂具有最佳的生化和生物力学特性，适合用作具有再生目的的纤维蛋白密封剂。

图形概要