Highly selective extraction of phosphopeptides is necessary before mass spectrometry (MS) analysis. Herein, zirconium phthalocyanine-modified magnetic nanoparticles were prepared through a simple method. The Fe–O groups on Fe₃O₄ and the zirconium ions on phthalocyanine had a strong affinity for phosphopeptides based on immobilized metal ion affinity chromatography (IMAC). The enrichment platform exhibited low detection limit (0.01 fmol), high selectivity (α-/β-casein/bovine serum albumin, 1/1/5000), good reusability (10 circles), and recovery (91.1 ± 1.1%) toward phosphopeptides. Nonfat milk, human serum, saliva, and A549 cell lysate were employed as actual samples to assess the applicability of the enrichment protocol. Metallo-phthalocyanine will be a competitive compound for designing highly efficient adsorbents and offers a new approach to phosphopeptide analysis.

中文翻译：

---

基于锆酞菁修饰磁性纳米粒子的磷酸肽富集

在进行质谱 (MS) 分析之前，必须高度选择性地提取磷酸肽。本文通过简单的方法制备了锆酞菁修饰的磁性纳米颗粒。根据固定化金属离子亲和色谱 (IMAC)， Fe ₃ O ₄上的 Fe-O 基团和酞菁上的锆离子对磷酸肽具有很强的亲和力。该富集平台对磷酸肽具有低检测限（0.01 fmol）、高选择性（α-/β-酪蛋白/牛血清白蛋白，1/1/5000）、良好的可重用性（10圈）和回收率（91.1 ± 1.1%） 。采用脱脂牛奶、人血清、唾液和 A549 细胞裂解物作为实际样品来评估富集方案的适用性。金属酞菁将成为设计高效吸附剂的有竞争力的化合物，并为磷酸肽分析提供新方法。