Repetitive transcranial magnetic stimulation (rTMS), a non-invasive brain stimulation technique, holds potential for applications in the treatment of Alzheimer’s disease (AD). This study aims to compare the therapeutic effects of rTMS at different frequencies on Alzheimer’s disease and explore the alterations in neuronal electrophysiological properties throughout this process. APP/PS1 AD mice were subjected to two rTMS treatments at 0.5 Hz and 20 Hz, followed by assessments of therapeutic outcomes through the Novel Object Recognition (NOR) and Morris Water Maze (MWM) tests. Following this, whole-cell patch-clamp techniques were used to record action potential, voltage-gated sodium channel currents, and voltage-gated potassium channel currents in dentate gyrus granule neurons. The results show that AD mice exhibit significant cognitive decline compared to normal mice, along with a pronounced reduction in neuronal excitability and ion channel activity. Both frequencies of rTMS treatment partially reversed these changes, demonstrating similar therapeutic efficacy. Furthermore, the investigation indicates that low-frequency magnetic stimulation inhibited the concentrated firing of early action potentials in AD.

中文翻译：

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低频 rTMS 调节阿尔茨海默病小鼠模型海马神经元的兴奋性和高频放电

重复经颅磁刺激（rTMS）是一种非侵入性脑刺激技术，在治疗阿尔茨海默病（AD）方面具有应用潜力。本研究旨在比较不同频率的rTMS对阿尔茨海默病的治疗效果，并探讨整个过程中神经元电生理特性的变化。 APP/PS1 AD 小鼠接受 0.5 Hz 和 20 Hz 两种 rTMS 治疗，然后通过新物体识别 (NOR) 和莫里斯水迷宫 (MWM) 测试评估治疗结果。随后，使用全细胞膜片钳技术记录齿状回颗粒神经元的动作电位、电压门控钠通道电流和电压门控钾通道电流。结果表明，与正常小鼠相比，AD 小鼠的认知能力显着下降，神经元兴奋性和离子通道活性也显着降低。两种频率的 rTMS 治疗都部分逆转了这些变化，表现出相似的治疗效果。此外，研究表明低频磁刺激抑制了 AD 早期动作电位的集中放电。