Determination of adulteration in raw milk has become increasingly important from the viewpoint of food safety and quality. The present study aimed to detect the adulteration of camel milk with cattle and goat milk by multiplex PCR technique based on amplification of the Cytochrome b gene. The developed technique successfully amplified the target fragment of 208 bp (Camel), 274 bp (cattle), and 174 bp (goat) of the gene. The amplified products were sequenced for further verification and submitted to NCBI GenBank. The limit of quantification (LOQ) for camel, cattle and goat were 2 × 10 ng μL, 2 × 10 ng μL and 2 × 10 ng μL respectively. The limit of detection (LOD) of cattle and goat milk in camel milk was found to be 10% and 5%, respectively. Results of the present study indicated that the multiplex PCR analysis is a useful technique for authentication of camel milk and detection of its adulteration with cattle and goat milk.

中文翻译：

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多重 PCR 检测骆驼奶与牛奶和羊奶的掺假

从食品安全和质量的角度来看，原奶掺假的判定变得越来越重要。本研究旨在通过基于细胞色素b基因扩增的多重PCR技术检测骆驼奶与牛奶和羊奶的掺假。所开发的技术成功扩增了该基因的208 bp（骆驼）、274 bp（牛）和174 bp（山羊）的目标片段。扩增产物进行测序以供进一步验证，并提交至 NCBI GenBank。骆驼、牛和山羊的定量限 (LOQ) 分别为 2 × 10 ng µL、2 × 10 ng µL 和 2 × 10 ng µL。骆驼奶中牛奶和羊奶的检测限 (LOD) 分别为 10% 和 5%。本研究结果表明，多重 PCR 分析是鉴定骆驼奶以及检测其与牛奶和羊奶掺假的有用技术。