Abstract

In this work, the glucose oxidase enzyme was immobilized in a sol-gel matrix based on dimethyldiethoxysilane and tetraethoxysilane in a volume ratio of 50/50%. Polyvinyl alcohol and chitosan were used as structure control agents. A number of physicochemical methods were used to study the morphology of the resulting immobilized biomaterials. The formation of organosilicon polymers was confirmed using IR spectroscopy. Differences in the morphology of the resulting sol-gel matrix structures were determined by SEM. The distribution of pores in samples of synthesized materials was determined using the method of low-temperature nitrogen adsorption. The catalytic activity of immobilized enzymes was studied using the biosensor method. Glucose oxidase is characterized by similar sensitivity characteristics when encapsulated in a sol-gel matrix using the structure-controlling agents chitosan and polyvinyl alcohol (sensitivity coefficients (3.8 × 10^–3 and 3.0 × 10^–3, mg O₂ min^–1 mmol^–1, respectively). However, the addition of chitosan to the matrix allows the performance of the enzyme biosensor to be increased up to 17 days, as well as reducing the relative standard deviation, thereby increasing the stability of the biosensor.

中文翻译：

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有机硅材料与结构控制剂组合作为葡萄糖氧化酶固定化的基础

摘要

在这项工作中，葡萄糖氧化酶被固定在基于体积比为50/50%的二甲基二乙氧基硅烷和四乙氧基硅烷的溶胶-凝胶基质中。使用聚乙烯醇和壳聚糖作为结构控制剂。使用许多物理化学方法来研究所得固定生物材料的形态。使用红外光谱证实了有机硅聚合物的形成。通过 SEM 测定所得溶胶-凝胶基质结构的形态差异。采用低温氮气吸附法测定了合成材料样品中的孔分布。采用生物传感器法研究了固定化酶的催化活性。当使用结构控制剂壳聚糖和聚乙烯醇封装在溶胶-凝胶基质中时，葡萄糖氧化酶具有相似的灵敏度特征（灵敏度系数（3.8 × 10 ^–3和 3.0 × 10 ^–3 , mg O ₂ min ^–1 mmol ^–然而，在基质中添加壳聚糖可以使酶生物传感器的性能提高长达17天，并降低相对标准偏差，从而提高生物传感器的稳定性。