It is crucial to enhance new compounds for the treatment of most malignancies, and graphene oxide/silver nanocomposite (GO/Ag NC) has been paying attention to biomedical applications such as malignancies. In this work, green synthesized Ag@Cht NPs were successfully produced using chitosan for reduction and stabilization and added on GO sheet forming novel GO/Ag NC. Then, the produced anticancer nanomaterials GO, Ag@Cht NPs, and GO/Ag NC were assessed for their cytotoxicity against four distinct cancer cell lines: H460, HCT116, MDA-MB-468, and FaDu cells, at varying concentrations, using SRP assay after 48 h. The prepared nanomaterials were characterized by TEM, UV–Vis spectrophotometry, FTIRs, Raman spectroscopy and XRD. TEM images showed a regular spread of Ag@Cht NPs on the GO sheets with an average particle size of 15 nm. UV–Vis spectrophotometry shows two main characteristic peaks for GO/Ag NC, one close to 230 nm corresponds to GO, while the other peak at 425 nm due to Ag@Cht decorating the GO surface was blue shifted by few nanometers from 427 nm for Ag@Cht. Results of the laser irradiation by DPSS (diode-pumped solid state) confirm the photothermal stability of the prepared nanocomposite as there is no change in surface plasmon resonance (SPR) with varying exposure time. FTIRs measurements indicate that Ag ions interact with a hydroxyl group. This interaction shifts the O–H wavenumber and decreases the bond stretching intensity. In addition, Ag@Cht NPs and Ag/GO NC showed enhanced activity against cancerous cells. Results showed that GO, Ag@Cht NPs, and GO/Ag NC at (200, 400, and 600 µg/ml) had an impact on all evaluated cell lines. In MDA-MB-468, HCT116, H460, and FaDu cells, Ag@Cht NPs had the most effect across all employed cell lines, with IC50 values of 5.5, 9, 6, and 7.75 µg/ml, respectively. In conclusion, the produced novel nanocomposite may be an effective way to treat different cell lines, and future work is to use the prepared nanomaterials as anticancer drug delivery in photothermal chemotherapy combination treatment.

中文翻译：

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新绿色合成的银/氧化石墨烯纳米复合材料对不同癌细胞系的抗肿瘤效率和光稳定性

增强新化合物对大多数恶性肿瘤的治疗至关重要，氧化石墨烯/银纳米复合材料（GO/Ag NC）一直关注恶性肿瘤等生物医学应用。在这项工作中，使用壳聚糖进行还原和稳定，成功生产了绿色合成的Ag@Cht NPs，并将其添加到GO片上形成新型GO/Ag NC。然后，使用 SRP 评估了所生产的抗癌纳米材料 GO、Ag@Cht NP 和 GO/Ag NC 对四种不同癌细胞系（H460、HCT116、MDA-MB-468 和 FaDu 细胞）在不同浓度下的细胞毒性。 48小时后测定。通过TEM、紫外-可见分光光度法、FTIR、拉曼光谱和X射线衍射对所制备的纳米材料进行了表征。TEM 图像显示 Ag@Cht NPs 在 GO 片上规则分布，平均粒径为 15 nm。紫外-可见分光光度法显示 GO/Ag NC 的两个主要特征峰，一个接近 230 nm 对应于 GO，而另一个 425 nm 处的峰由于 Ag@Cht 装饰 GO 表面而从 427 nm 蓝移了几纳米。 Ag@Cht。DPSS（二极管泵浦固态）激光照射的结果证实了所制备的纳米复合材料的光热稳定性，因为随着曝光时间的变化，表面等离子共振（SPR）没有变化。FTIR 测量表明银离子与羟基相互作用。这种相互作用改变了 O-H 波数并降低了键拉伸强度。此外，Ag@Cht NPs 和 Ag/GO NC 显示出增强的抗癌细胞活性。结果显示，GO、Ag@Cht NP 和 GO/Ag NC（200、400 和 600 µg/ml）对所有评估的细胞系都有影响。在 MDA-MB-468、HCT116、H460 和 FaDu 细胞中，Ag@Cht NP 在所有使用的细胞系中效果最强，IC50 值分别为 5.5、9、6 和 7.75 µg/ml。总之，所生产的新型纳米复合材料可能是治疗不同细胞系的有效方法，未来的工作是将所制备的纳米材料用作光热化疗联合治疗中的抗癌药物递送。