Medicinal applications of Plumbago zeylanica and its metabolites on various diseases and low viability and inconsistent germination of its seeds are the reasons behind the loss of its genetic diversity. Hence, an efficient protocol for the short-term storage of P. zeylanica synthetic seeds, which is an overexploited medicinally valuable plant, was developed. Initially, in vitro culture was performed from nodal explants to develop synthetic seeds from its proliferated shoots. Murashige and Skoog (MS) medium augmented with 0.5 mg L⁻¹ 6-benzylaminopurine (BAP) resulted in the best morphogenetic response. Thereafter, the developed synseeds were stored for 2 wk at a temperature of 10 or 25°C in different conditions and further evaluated for regeneration. Higher re-growth rate (80%) and the identical morphogenetic response were recorded for the P. zeylanica synthetic seeds, which were stored at a temperature of 10°C in dark condition after its storage period. As per the available literature, this is the first report pertaining to in vitro low-temperature storage of synthetic seeds of P. zeylanica and can further be utilized for the conservation of elite clones for the study of medicinally potent species.

白花 丹及其代谢物对多种疾病的药用应用以及其种子活力低下和发芽不一致是其遗传多样性丧失的原因。因此，开发了一种短期储存P. zeylanica合成种子（一种过度开发的具有药用价值的植物）的有效方案。最初，从节外植体进行体外培养，以从其增殖的芽中发育合成种子。^{添加 0.5 mg L -1} 6-苄氨基嘌呤 (BAP)的 Murashige 和 Skoog (MS) 培养基产生了最佳的形态发生反应。此后，将发育好的合成种子在10°C或25°C的温度下在不同条件下储存2周，并进一步评估再生情况。P. zeylanica合成种子记录了更高的再生长率 (80%) 和相同的形态发生反应，这些种子在储存期结束后在 10°C 的温度下在黑暗条件下储存。根据现有文献，这是第一份关于P. zeylanica合成种子体外低温储存的报告，可进一步用于保存优良克隆，以研究药用有效物种。