Continuing productivity of the acclimatization stage of plantlets means successful micropropagation. Due to the free water and high humidity in the culture container environment, poorly developed cuticle results in rapid water loss and drying of plantlets with watery stems and leaves, incomplete stomata, and large intercellular space. When plantlets are exposed to an environmental condition different from the culture medium, problems, such as rapid water loss and drying, may be encountered, and the survival rate of plantlets in vitro may be reduced. The aim of this study was to reduce the stress factors that occur during the acclimatization phase. For this reason, various healing agents have been used. Some of these compounds are ascorbic acid (AsA), salicylic acid (SA), and sodium nitroprusside (SNP). In the study, the response of AsA (100.0 and 200.0 mg L⁻¹), SA (100.0 and 200.0 mg L⁻¹), and SNP (100.0 and 200.0 µM) applications on growth parameters (survival rate (%), node count (pieces per plant), count of leaves (pieces per plant), shoot length (mm), and shoot diameter (mm)) and physiological variable (Soil Plant Analysis Development (SPAD)) were examined. The AsA100 (100.0 mg L⁻¹ ascorbic acid), AsA200 (200.0 mg L⁻¹ ascorbic acid), and SNP100 (100.0 µM sodium nitroprusside) applications resulted in an improvement in node count, leaf count per plant, shoot length, and shoot diameter parameters. The highest shoot length (60.50 ± 7.81 mm) and node count (16.83 ± 1.15 pieces per plantlet) were achieved with the AsA100 application. The maximum stem diameter (2.31 ± 0.37 mm) was determined with the SNP100 application. There were no statistically significant differences found in the survival rate, leaf count, and SPAD parameter. The current study determined that AsA, SA, and SNP applications were effective in regulating several growth parameters in Garnem plantlets and in reducing acclimation stress thereby facilitating adaptation to external conditions.

幼苗适应阶段的持续生产力意味着微繁殖的成功。由于培养容器环境中存在游离水，湿度高，角质层发育不良，导致植株失水快，茎叶水润，气孔不完整，细胞间隙大。当试管苗暴露于与培养基不同的环境条件时，可能会遇到水分快速流失、干燥等问题，导致试管苗的体外成活率降低。本研究的目的是减少适应阶段发生的压力因素。为此，使用了各种治疗剂。其中一些化合物是抗坏血酸 (AsA)、水杨酸 (SA) 和硝普钠 (SNP)。在研究中，AsA（100.0和200.0 mg L ^-1）、SA（100.0和200.0 mg L ^-1）和SNP（100.0和200.0 µM）应用对生长参数（存活率（％）、节点数）的响应检查了（每株植物的片数）、叶数（每株植物的片数）、枝条长度（毫米）和枝条直径（毫米））和生理变量（土壤植物分析开发（SPAD））。AsA100（100.0 mg L ^-1抗坏血酸）、AsA200（200.0 mg L ^-1抗坏血酸）和 SNP100（100.0 µM 硝普钠）的应用导致节数、单株叶数、芽长和芽数的改善直径参数。使用 AsA100 获得了最高的枝条长度（60.50 ± 7.81 毫米）和节数（每株植株 16.83 ± 1.15 个）。最大茎直径 (2.31 ± 0.37 mm) 通过 SNP100 应用程序确定。存活率、叶数和SPAD参数没有统计学上的显着差异。目前的研究确定，AsA、SA 和 SNP 的应用可有效调节 Garnem 幼苗的多个生长参数，并减少驯化应激，从而促进对外部条件的适应。