Real-time monitoring of intestinal movement is critical for the clinical diagnosis of bowel diseases, however, the application of organic fluorophores in non-invasive imaging of the intestinal tract via systemic administration is still challenging. We herein report a series of albumin-chaperoned chloro-cyanine dyes for high spatiotemporal tracking of intestinal peristalsis and bowel disease in vivo. By adjusting the size of the central rings, , with a five-membered ring and meso-chlorine, could covalently bind with circulating endogenous albumin in the blood, generating a bright and stable cyanine-albumin fluorescent complex with ∼1055 nm fluorescence in the near-infrared-II window in situ. Notably, was able to clearly view intestinal peristalsis by harnessing the inherent intestine tropism of -albumin complex following intravenous (i.v.) administration. Furthermore, a hydroxyl radical (·OH)-activatable fluorophore () was created, allowing for the selective detection of overproduced ·OH in bowel disease as well as the successful visualization of the inflammatory intestinal tract in vivo after i.v. injection. This work provides a facile strategy for the investigation of intestinal movement and related diseases.

中文翻译：

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通过原位生成的 NIR-II 花青白蛋白复合物对肠道蠕动和肠道疾病进行高时空追踪

肠道运动的实时监测对于肠道疾病的临床诊断至关重要，然而，有机荧光团通过全身给药在肠道无创成像中的应用仍然具有挑战性。我们在此报告了一系列白蛋白陪伴的氯菁染料，用于体内肠道蠕动和肠道疾病的高时空追踪。通过调整中心环的大小，具有五元环和内消旋氯的 可以与血液中循环的内源性白蛋白共价结合，产生明亮且稳定的花青-白蛋白荧光复合物，在近处具有～1055 nm的荧光。 - 红外-II 窗口原位。值得注意的是，通过静脉内 (iv) 给药后利用β-白蛋白复合物固有的肠道向性，能够清楚地观察肠道蠕动。此外，还创建了羟自由基（·OH）可激活的荧光团（），可以选择性检测肠道疾病中过量产生的·OH，并在静脉注射后成功观察体内炎症肠道。这项工作为研究肠道运动和相关疾病提供了一种简便的策略。