Wheat bran is an abundant source of starch fragments, peptides and amino acids which represent a valuable nutrient source for bacterial production of natural products. The commercial protease Corolase® 7089 efficiently improves extraction of N-compounds from wheat bran. Here we show the effect of combined applications with the commercial α-amylase Termamyl® 120. Wheat bran was extracted with at a liquid/solid ratio of 12.3. The same product yield of 24% was obtained after simultaneous enzyme application for 1 h and after serial application (first amylase) with a total treatment time of 7.75 h. Crude protein concentration in the product was 23% and 22%, respectively. Measurements of the sugar concentration in the extraction liquid indicate that the degree of starch hydrolysis was decreased in the presence of protease. In a stirred pilot-scale reactor a product yield of 32% was achieved.

中文翻译：

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麦麸提取物作为生物技术过程中氨基化合物和糖的来源 

麦麸是淀粉碎片、肽和氨基酸的丰富来源，是细菌生产天然产物的宝贵营养来源。商用蛋白酶 Corolase® 7089 可有效提高麦麸中氮化合物的提取。在这里，我们展示了与商业 α-淀粉酶 Termamyl® 120 联合应用的效果。以液/固比为 12.3 提取麦麸。同时酶应用 1 小时和连续应用酶（第一次淀粉酶）总处理时间为 7.75 小时后，获得了 24% 的相同产品产率。产品中粗蛋白浓度分别为23%和22%。提取液中糖浓度的测量表明，在蛋白酶存在下，淀粉水解程度降低。在搅拌式中试规模反应器中，产物收率为 32%。