Particles with a porous structure can lead to quick hemostasis and provide a good matrix for cell proliferation during wound healing. Recently, many particle-based wound healing materials have been clinically applied. However, these products show good hemostatic ability but with poor wound healing ability. To solve this problem, this study fabricated APGG composite particles using yeast β-glucan (obtained from Saccharomyces cerevisiae), sodium alginate, and γ-polyglutamic acid as the starting materials. The structure of yeast β-glucan was modified with many carboxymethyl groups to obtain carboxymethylated β-glucan, which could coordinate with Ca²⁺ ions to form a crosslinked structure. A morphology study indicated that the APGG particles showed an irregular spheroidal structure with a low density (<0.1 g cm⁻³) and high porosity (>40%). An in vitro study revealed that the particles exhibited a low BCI value, low hemolysis ratio, and good cytocompatibility against L929 cells. The APGG particles could quickly stop bleeding in a mouse liver injury model and exhibited better hemostatic ability than the commercially available product Celox. Furthermore, the APGG particles could accelerate the healing of non-infected wounds, and the expression levels of CD31, α-SMA, and VEGF related to angiogenesis were significantly enhanced.

中文翻译：

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止血促愈合酵母β-葡聚糖/海藻酸钠/γ-聚谷氨酸复合颗粒的制备

具有多孔结构的颗粒可以导致快速止血，并为伤口愈合过程中的细胞增殖提供良好的基质。近年来，许多基于颗粒的伤口愈合材料已应用于临床。然而，这些产品显示出良好的止血能力，但伤口愈合能力较差。为了解决这个问题，本研究以酵母β-葡聚糖（从酿酒酵母中获得）、海藻酸钠和γ-聚谷氨酸为起始原料，制备了APGG复合颗粒。对酵母β-葡聚糖的结构进行多个羧甲基修饰，得到羧甲基化β-葡聚糖，其可与Ca ²⁺离子配位形成交联结构。形态研究表明APGG颗粒呈现出不规则的球状结构，具有低密度(<0.1 g cm ^-3 )和高孔隙率(>40%)。体外研究表明，该颗粒具有低BCI值、低溶血率和对L929细胞良好的细胞相容性。APGG颗粒可以在小鼠肝损伤模型中快速止血，并且比市售产品Celox表现出更好的止血能力。此外，APGG颗粒可以加速非感染伤口的愈合，与血管生成相关的CD31、α-SMA和VEGF的表达水平显着增强。