Lactic acid bacteria (LAB) can be used as a probiotic or starter culture in dairy, meat, and vegetable fermentation. Therefore, their isolation and identification are essential. Recent advances in omics technologies and high-throughput sequencing have made the identification and characterization of bacteria. This study firstly aimed to demonstrate the sensitivity of the Vitek MS (MALDI-TOF) system in the identification of lactic acid bacteria and, secondly, to characterize bacteria using various bioinformatics approaches. Probiotic potency-related genes and secondary metabolite biosynthesis gene clusters were examined. The Vitek MS (MALDI-TOF) system was able to identify all of the bacteria at the genus level. According to whole genome sequencing, the bacteria were confirmed to be Lentilactobacillus buchneri, Levilactobacillus brevis, Lactiplantibacillus plantarum, Levilactobacillus namurensis. Bacteria had most of the probiotic potency-related genes, and different toxin-antitoxin systems such as PemIK/MazEF, Hig A/B, YdcE/YdcD, YefM/YoeB. Also, some of the secondary metabolite biosynthesis gene clusters, some toxic metabolite-related genes, and antibiotic resistance-related genes were detected. In addition, Lentilactobacillus buchneri Egmn17 had a type II-A CRISPR/Cas system. Lactiplantibacillus plantarum Gmze16 had a bacteriocin, plantaricin E/F.

乳酸菌 (LAB) 可用作乳制品、肉类和蔬菜发酵中的益生菌或发酵剂。因此，对其进行隔离和鉴定至关重要。组学技术和高通量测序的最新进展已经实现了细菌的鉴定和表征。本研究首先旨在证明 Vitek MS (MALDI-TOF) 系统在鉴定乳酸菌方面的敏感性，其次是使用各种生物信息学方法来表征细菌。检查了益生菌效力相关基因和次级代谢物生物合成基因簇。Vitek MS (MALDI-TOF) 系统能够在属水平上识别所有细菌。根据全基因组测序，确认该细菌为布赫内里慢杆菌（Lentilactobacillus buchneri）、短乳杆菌（Levilactobacillus brevis）、植物乳杆菌（Lactiplantibacillus plantarum）、纳穆伦杆菌（Levilactobacillus namurensis）。细菌拥有大部分益生菌效力相关基因，以及不同的毒素-抗毒素系统，如PemIK/MazEF、Hig A/B、YdcE/YdcD、YefM/YoeB。此外，还检测到一些次生代谢物生物合成基因簇、一些有毒代谢物相关基因和抗生素耐药性相关基因。此外，布赫内里扁豆乳杆菌Egmn17 具有 II-A 型 CRISPR/Cas 系统。植物乳杆菌Gmze16 具有细菌素，植物素 E/F。