: Afterglow materials with organic room temperature phosphorescence (RTP) or thermally activated delayed fluorescence (TADF) exhibit significant potential in biological imaging due to their long lifetime. By utilizing time-resolved technology, interference from biological tissue fluorescence can be mitigated, enabling high signal-- to-background ratio imaging. Despite the continued emergence of individual reports on RTP or TADF in recent years, comprehensive reviews addressing these two materials are rare. Therefore, this review aims to provide a comprehensive overview of several typical molecular designs for organic RTP and TADF materials. It also explores the primary methods through which triplet excitons resist quenching by water and oxygen. Furthermore, we analyze the principal challenges faced by afterglow materials and discuss key directions for future research with the hope of inspiring developments in afterglow imaging.

中文翻译：

---

调节有机发光体的三重态激子以促进生物成像

：具有有机室温磷光（RTP）或热激活延迟荧光（TADF）的余辉材料由于其长寿命而在生物成像中表现出巨大的潜力。通过利用时间分辨技术，可以减轻生物组织荧光的干扰，从而实现高信号背景比成像。尽管近年来关于 RTP 或 TADF 的单独报告不断出现，但针对这两种材料的全面评论却很少。因此，本综述旨在全面概述有机 RTP 和 TADF 材料的几种典型分子设计。它还探索了三重态激子抵抗水和氧气猝灭的主要方法。此外，我们分析了余辉材料面临的主要挑战，并讨论了未来研究的关键方向，希望能够激发余辉成像的发展。