Postsurgical treatment comprehensively benefits from the application of tissue-adhesive injectable hydrogels, which reduce postoperative complications by promoting wound closure and tissue regeneration. Although various hydrogels have been employed as clinical tissue adhesives, many exhibit deficiencies in adhesive strength under wet conditions or in immunomodulatory functions. Herein, we report the development of reactive oxygen species (ROS) scavenging and tissue-adhesive injectable hydrogels composed of polyamine-modified gelatin crosslinked with the 4-arm poly (ethylene glycol) crosslinker. Polyamine-modified gelatin was particularly potent in suppressing the secretion of proinflammatory cytokines from stimulated primary macrophages. This effect is attributed to its ability to scavenge ROS and inhibit the nuclear translocation of nuclear factor kappa-B. Polyamine-modified gelatin-based hydrogels exhibited ROS scavenging abilities and enhanced tissue adhesive strength on collagen casing. Notably, the hydrogel demonstrated exceptional tissue adhesive properties in a wet environment, as evidenced by its performance using porcine small intestine tissue. This approach holds significant promise for designing immunomodulatory hydrogels with superior tissue adhesion strength compared to conventional medical materials, thereby contributing to advancements in minimally invasive surgical techniques.

中文翻译：

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通过寡乙烯亚胺的蛋白质修饰增强可注射水凝胶的 ROS 清除和组织粘附能力

术后治疗全面受益于组织粘附注射水凝胶的应用，它通过促进伤口闭合和组织再生来减少术后并发症。尽管各种水凝胶已被用作临床组织粘合剂，但许多水凝胶在潮湿条件下的粘合强度或免疫调节功能方面表现出缺陷。在此，我们报告了活性氧（ROS）清除和组织粘附可注射水凝胶的开发，该水凝胶由与四臂聚（乙二醇）交联剂交联的聚胺改性明胶组成。多胺修饰明胶在抑制受刺激的初级巨噬细胞分泌促炎细胞因子方面特别有效。这种作用归因于其清除 ROS 和抑制核因子 kappa-B 核转位的能力。聚胺改性明胶基水凝胶表现出 ROS 清除能力并增强胶原蛋白外壳上的组织粘合强度。值得注意的是，该水凝胶在潮湿环境中表现出卓越的组织粘合性能，其使用猪小肠组织的性能证明了这一点。与传统医用材料相比，这种方法对于设计具有优异组织粘附强度的免疫调节水凝胶具有重大前景，从而有助于微创手术技术的进步。