Purpose

Adding organic soil amendments, like animal manure, can alleviate the mobility of lead (Pb). However, there is a scarcity of in vivo studies examining the impact of animal manure as a soil amendment on mammals.

Methods

Six C57BL/6 mice per group were raised for 30 and 98 days on soil containing 3000 mg Pb/kg. On Pb-spiked soil, chicken manure (40 t/ha) was applied as an immobilizer. Pb concentrations in the tissues were analyzed using ICP-MS after microwave digestion. RT-PCR was used to measure the mRNA expression of antioxidant and cell apoptosis genes in the liver, kidneys, and brain.

Results

When chicken manure was used as the immobilizer in Pb-spiked soil, Pb concentrations in the brain, kidneys, and lungs at day 30 were significantly different (p < 0.05) with reduction percentages of 28.58, 26.63, and 25.62, respectively. The brain, kidneys, liver, and bone, except for the lungs and trachea, also showed a similar reducing phenomenon on day 98, with percentages of 11.82, 4.63, 29.87, and 14.54, respectively. There was a significant difference in the levels of Nrf2, HMOX1, SOD1, CAT, BCL2, and Bax in the brain between Pb-exposed and remediated groups after 98 days of exposure.

Conclusions

This study highlights that the utilization of chicken manure reduced Pb accumulation in tissues (6.25–28.58% at 30 days and 4.63–29.87% at 98 days). This reduction in Pb accumulation subsequently alleviated the body burden by activating antioxidant genes in the brain, notably HMOX1, SOD1, and CAT.

中文翻译：

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鸡粪改良剂对小鼠铅负荷的影响：暴露于加铅土壤

目的

添加有机土壤改良剂（例如动物粪便）可以减轻铅 (Pb) 的迁移性。然而，缺乏体内研究来检验动物粪便作为土壤改良剂对哺乳动物的影响。

方法

每组六只 C57BL/6 小鼠在含 3000 mg Pb/kg 的土壤中饲养 30 天和 98 天。在加铅土壤上，施用鸡粪（40 吨/公顷）作为固定剂。微波消解后使用 ICP-MS 分析组织中的 Pb 浓度。RT-PCR用于测量肝脏、肾脏和大脑中抗氧化和细胞凋亡基因的mRNA表达。

结果

当鸡粪用作加铅土壤中的固定剂时，第30天时脑、肾和肺中的Pb浓度存在显着差异（p  < 0.05），降低百分比分别为28.58、26.63和25.62。除肺和气管外，脑、肾、肝和骨在第98天也出现类似的减少现象，百分比分别为11.82、4.63、29.87和14.54。暴露98天后，铅暴露组和修复组大脑中Nrf2、HMOX1、SOD1、CAT、BCL2和Bax的水平存在显着差异。

结论

这项研究强调，鸡粪的利用减少了组织中的铅积累（30 天时减少 6.25-28.58%，98 天时减少 4.63-29.87%）。Pb 积累的减少随后通过激活大脑中的抗氧化基因（尤其是 HMOX1、SOD1 和 CAT）减轻了身体负担。