Purpose

The purpose of this study was to provide a detailed analysis of clinical and laboratory factors associated with skewed secondary sex ratio (SSR) after ART.

Method

Retrospective cohort study of embryos resulting in live births, from frozen and fresh single blastocyst transfers. Embryos were cultured in either G-TL (n = 686) or Sage media (n = 685). Data was analyzed using a multivariate logistic regression model and a mixed model analysis.

Results

Significantly more male singletons were born after culture in Sage media compared to G-TL media (odds ratio (OR) 1.34, 95% CI (1.05, 1.70), P = 0.02). Inner cell mass grade B vs A (OR 1.36 95% CI (1.05, 1.76), P = 0.02) and one previous embryo transfer (OR 1.49, 95% CI (1.03, 2.16), P = 0.03) were associated with a significantly higher probability of male child at birth. Factors associated with a reduced probability of male child were expansion grade 3 vs 5 (OR 0.66, 95% CI (10.45, 0.96), P = 0.03) and trophectoderm grade B vs A (OR 0.57, 95% CI (0.44, 0.74), P = 0.00). Male embryos developed significantly faster in Sage media compared to G-TL media for the stages of blastocyst (− 1.12 h, 95% CI (− 2.12, − 0.12)), expanded blastocyst (− 1.35 h, 95% CI (− 2.34, − 0.35)), and hatched blastocyst (− 1.75 h, 95% CI (− 2.99, − 0.52)).

Conclusion

More male children were born after culture in Sage media compared to G-TL media. Male embryo development was affected by culture media. Our observations suggest that culture media impact male embryo quality selectively, thus potentially favoring the selection of male embryos.

中文翻译：

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培养基影响 IVF 治疗后的性行为——解释变量的详细分析

目的

本研究的目的是对与 ART 后第二性别比 (SSR) 偏差相关的临床和实验室因素进行详细分析。

方法

对通过冷冻和新鲜单囊胚移植产生活产的胚胎进行回顾性队列研究。胚胎在 G-TL ( n  = 686) 或 Sage 培养基 ( n = 685)中培养 。使用多元逻辑回归模型和混合模型分析来分析数据。

结果

与 G-TL 培养基相比，Sage 培养基培养后出生的雄性单胎明显更多（优势比 (OR) 1.34，95% CI (1.05, 1.70)，P  = 0.02）。内细胞质量 B 级与 A 级（OR 1.36 95% CI (1.05, 1.76)，P  = 0.02）和之前一次胚胎移植（OR 1.49，95% CI (1.03, 2.16)，P  = 0.03）与显着相关出生时生男孩的概率较高。与男孩概率降低相关的因素是扩张3级与5级（OR 0.66，95％CI（10.45，0.96），P  = 0.03）和滋养外胚层B级与A级（OR 0.57，95％CI（0.44，0.74） ，P  = 0.00）。与 G-TL 培养基相比，雄性胚胎在囊胚阶段 (− 1.12 h，95% CI (− 2.12，− 0.12))、扩张囊胚阶段 (− 1.35 h，95% CI (− 2.34， − 0.35)) 和孵化的囊胚 (− 1.75 小时，95% CI (− 2.99, − 0.52))。

结论

与 G-TL 培养基相比，Sage 培养基培养后出生的男婴更多。雄性胚胎发育受到培养基的影响。我们的观察表明，培养基选择性地影响雄性胚胎质量，因此可能有利于雄性胚胎的选择。