This study investigates the response of lentil genotypes to blight disease over two cropping years, 2021 and 2022. In addition, this research aims to assess the marker-trait association between disease progress and the selected SSRs. Visual symptoms were assessed using Hashemi's disease scoring system, leading to the calculation of disease parameters such as Percent Disease Index (PDI) and Area Under Disease Progress Curve (AUDPC). The results revealed variations in disease progress among genotypes, with some exhibiting low disease indices, particularly genotype IC241072. Genetic diversity analysis using SSR markers identified 30 alleles and grouped genotypes into four clusters. Principal Coordinate Analysis (PCoA) confirmed the genetic distances among genotypes. Marker-trait association analysis linked the microsatellite locus LCSSR04_130362_118bp to blight resistance, supported by the identification of a TCP family transcription factor in close proximity. The validation of the association indicated a significant difference between resistant and susceptible alleles. This comprehensive approach combining field screening, genetic diversity analysis, and marker-trait associations provides valuable insights into the genetic basis of Stemphylium blight resistance in lentil genotypes, offering potential candidates for future breeding programs.

中文翻译：

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标记性状关联分析揭示与扁豆茎叶菌病反应相关的基因组区域

本研究调查了 2021 年和 2022 年两个种植年中扁豆基因型对枯萎病的反应。此外，本研究旨在评估疾病进展与所选 SSR 之间的标记-性状关联。使用哈希米疾病评分系统评估视觉症状，从而计算疾病参数，例如疾病百分比指数 (PDI) 和疾病进展曲线下面积 (AUDPC)。结果揭示了基因型之间疾病进展的差异，其中一些表现出较低的疾病指数，特别是基因型 IC241072。使用 SSR 标记进行遗传多样性分析，确定了 30 个等位基因，并将基因型分为四个簇。主坐标分析（PCoA）证实了基因型之间的遗传距离。标记性状关联分析将微卫星基因座 LCSR04_130362_118bp 与枯萎病抗性联系起来，并得到了附近 TCP 家族转录因子的鉴定的支持。关联的验证表明抗性和易感等位基因之间存在显着差异。这种综合方法结合了田间筛选、遗传多样性分析和标记性状关联，为扁豆基因型中茎叶枯病抗性的遗传基础提供了有价值的见解，为未来的育种计划提供了潜在的候选者。