A multifunctional probe based on ferrocenyl chalcone (Probe M) was designed and successfully synthesized and found to recognize tryptophan (Trp) and ascorbic acid (AA) in solution simultaneously. The minimum detection limit of Probe M for Trp-and AA was 1.3188 × 10 mol/L and 1.1545 × 10 mol/L, respectively. The binding ratios of Probe M to Trp-and AA were obtained by job plot as 1:1 and 2:1, respectively. According to the Benesi-Hildebrand equation, the complexation constants of Probe M for Trp-and AA were 3.0025 × 10 L/mol and 4.6606 × 10 L/mol, respectively. The binding mechanism was also investigated by IR and NMR titration.

中文翻译：

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基于二茂铁基查耳酮的多功能探针，用于检测溶液中的色氨酸和抗坏血酸

设计并成功合成了基于二茂铁基查耳酮的多功能探针（探针M），该探针可以同时识别溶液中的色氨酸（Trp）和抗坏血酸（AA）。 Probe M对Trp-和AA的最低检测限分别为1.3188×10 mol/L和1.1545×10 mol/L。通过工作图获得探针M与Trp-和AA的结合比分别为1:1和2:1。根据Benesi-Hildebrand方程，探针M对Trp-和AA的络合常数分别为3.0025×10 L/mol和4.6606×10 L/mol。还通过红外和核磁共振滴定研究了结合机制。