Dysregulation of covalently closed circular RNAs (circRNAs) has been associated with neurological disorders, the role of circHIVP2 in Parkinson's disease (PD) and its molecular mechanism is not well understood. 127 patients with PD and 85 healthy people were enrolled. RT-qPCR was employed to examine the levels of circHIVEP2. ROC curve to explore the diagnostic. Mpp induced the SH-SY5Y to construct an in vitro PD cell model. Cell viability, apoptosis, and secretion levels of inflammatory factors were analyzed by CCK-8, flow cytometry, and ELISA assay. CircHIVEP2 targets miRNA predicted by bioinformatics database and validated by the dual luciferase reporter and RIP assays. CircHIVEP2 was typically lower in PD patients than in controls. CircHIVEP2 has certain specificity and sensitivity to recognize PD patients from healthy individuals. miR-485-3p, a target miRNA of circHIVEP2, was significantly elevated in PD patients. Additionally, MPP induction reduced cell viability and promoted apoptosis and inflammatory factor overproduction. However, overexpression of circHIVEP2 significantly inhibited the effects of MPP, but this inhibition was significantly attenuated by elevated miR-485-3p. circHIVEP2 is a potential diagnostic biomarker for PD, and its upregulation mitigated MPP-induced nerve damage and inflammation and this may be through targeted by the miR-485-3p.

中文翻译：

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CircHIVEP2 通过靶向 miR-485-3p 减轻帕金森病的神经损伤和炎症反应

共价闭合环状 RNA (circRNA) 的失调与神经系统疾病有关，circHIVP2 在帕金森病 (PD) 中的作用及其分子机制尚不清楚。 127 名 PD 患者和 85 名健康人入组。采用 RT-qPCR 检测 circHIVEP2 的水平。 ROC曲线探索诊断。 Mpp诱导SH-SY5Y构建体外PD细胞模型。通过 CCK-8、流式细胞术和 ELISA 分析细胞活力、凋亡和炎症因子的分泌水平。 CircHIVEP2 靶向由生物信息学数据库预测的 miRNA，并通过双荧光素酶报告基因和 RIP 检测进行验证。 PD 患者中的 CircHIVEP2 通常低于对照组。 CircHIVEP2对于识别PD患者和健康个体具有一定的特异性和敏感性。 miR-485-3p（circHIVEP2 的靶 miRNA）在 PD 患者中显着升高。此外，MPP 诱导降低了细胞活力并促进细胞凋亡和炎症因子过量产生。然而，circHIVEP2 的过度表达显着抑制 MPP 的作用，但这种抑制会因 miR-485-3p 升高而显着减弱。 circHIVEP2 是 PD 的潜在诊断生物标志物，其上调可减轻 MPP 诱导的神经损伤和炎症，这可能是通过 miR-485-3p 靶向的。