Tissue engineered skin equivalents are increasingly recognized as potential alternatives to traditional skin models such as human skin or animal skin models. However, most of the currently investigated human skin equivalents (HSEs) are constructed using mammalian collagen which can be expensive and difficult to extract. Fish skin is a waste product produced by fish processing industries and identified as a cost-efficient and sustainable source of type I collagen. In this work, we describe a method for generating highly stable HSEs based on fibrin fortified tilapia fish collagen. The fortified fish collagen (FFC) formulation is optimized to enable reproducible fabrication of full-thickness HSEs that undergo limited contraction, facilitating the incorporation of human donor-derived skin cells and formation of biomimetic dermal and epidermal layers. The morphology and barrier function of the FFC HSEs are compared with a commercial skin model and validated with immunohistochemical staining and transepithelial electrical resistance testing. Finally, the potential of a high throughput screening platform with FFC HSE is explored by scaling down its fabrication to 96-well format.

中文翻译：

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基于鱼胶原蛋白生成高度稳定的人体皮肤等同物的可持续策略

组织工程皮肤等效物越来越被认为是人类皮肤或动物皮肤模型等传统皮肤模型的潜在替代品。然而，目前研究的大多数人类皮肤等效物 (HSE) 都是使用哺乳动物胶原蛋白构建的，这种胶原蛋白价格昂贵且难以提取。鱼皮是鱼类加工业产生的废物，被认为是一种经济高效且可持续的 I 型胶原蛋白来源。在这项工作中，我们描述了一种基于纤维蛋白强化罗非鱼胶原蛋白生成高度稳定的 HSE 的方法。强化鱼胶原蛋白 (FFC) 配方经过优化，可重复制造收缩有限的全层 HSE，促进人类供体皮肤细胞的融入以及仿生真皮和表皮层的形成。将 FFC HSE 的形态和屏障功能与商业皮肤模型进行比较，并通过免疫组织化学染色和跨上皮电阻测试进行验证。最后，通过将其制造规模缩小至 96 孔格式，探索了采用 FFC HSE 的高通量筛选平台的潜力。