The fluorescent flexible sensor for point-of-care quantification of clinical anticoagulant drug, Heparin (Hep), is still an urgent need of breakthrough. In this research, a hyperbranched poly(amido amine) (HPA) was decorated with tetraphenylethene (TPE) and Rhodamine B (RhB), constructing a ratiometric fluorescent sensor (TR-HPA) for Hep. When the sensor was exposed to Hep, the TPE units within the probe skeleton would aggregate, resulting in an increasing fluorescent emission at 483 nm. The 580 nm of fluorescence came from RhB enhance, simultaneously, due to the fluorescence resonance energy transfer. As a result, there are two good linear correlation between the fluorescence emission ratio (E/E) of TR-HPA and the Hep concentration over a range of 0–1.0 μM, with a low limit of detection of 3.0 nM. Furthermore, we incorporate the TR-HPA probe into a polyvinyl alcohol (PVA) hydrogel matrix to create a flexible fluorescent sensing system platform, denoted as TR-HPA/PVA. This approach offers a straightforward visual detection method by causing a fluorescence color change from pink to blue when trace amounts of Hep are present. The hydrogel-based fluorescent sensor streamlines the detection procedures for Hep in biomedical applications. It shows great potential in rapid and point-of-care human blood clotting condition monitoring, making it suitable for next-generation wearable medical devices.

中文翻译：

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基于水凝胶的比率荧光传感器，依靠罗丹明 B 标记的 AIE 特征的超支化聚（酰氨基胺）进行肝素检测

用于临床抗凝药物肝素（Hep）即时定量的荧光柔性传感器仍然迫切需要突破。在这项研究中，超支化聚酰胺胺（HPA）被四苯乙烯（TPE）和罗丹明B（RhB）修饰，构建了Hep的比率荧光传感器（TR-HPA）。当传感器暴露于 Hep 时，探针骨架内的 TPE 单元会聚集，导致 483 nm 处的荧光发射增加。 580 nm 的荧光来自于 RhB 增强，同时由于荧光共振能量转移。因此，TR-HPA的荧光发射比（E/E）与Hep浓度在0-1.0 μM范围内存在两个良好的线性相关性，检测下限为3.0 nM。此外，我们将 TR-HPA 探针融入聚乙烯醇（PVA）水凝胶基质中，以创建灵活的荧光传感系统平台，表示为 TR-HPA/PVA。这种方法提供了一种直接的视觉检测方法，当存在微量 Hep 时，荧光颜色会从粉红色变为蓝色。基于水凝胶的荧光传感器简化了生物医学应用中 Hep 的检测程序。它在快速和即时人体凝血状况监测方面显示出巨大潜力，使其适合下一代可穿戴医疗设备。