Beta-(1,3)(1,6)-D-glucan is a complex polysaccharide, which is found in the cell wall of various fungi, yeasts, bacteria, algae, barley, and oats and has immunomodulatory, anticancer and antiviral effects. In the present study, we investigated the effect of beta-(1,3)(1,6)-D-glucan derived from yeast on the proliferation of primary NK cells and breast cancer cell lines in 2D and 3D models, and on the cytotoxicity of primary NK cells against breast cancer cell lines in 2D and 3D models. In this study, we investigated the effects of different concentrations of yeast-derived beta-(1→3)(1→6)-D-glucan on the proliferation and cytotoxicity of human NK cells and breast cancer cell lines in 2D and 3D models using the XTT cell proliferation assay and the CellTiter-Glo® 2.0 assay to determine the cytotoxicity of human NK cells on breast cancer cell lines in 2D and 3D models. We found that the co-incubation of NK cells with beta-glucan in the absence of IL2 at 48 h significantly increased the proliferation of NK cells, whereas the co-incubation of NK cells with beta-glucan in the presence of IL2 (70 U/ml) increased the proliferation of NK cells but not significantly. Moreover, beta-glucan significantly inhibited the proliferation of breast cancer cell lines in 2D model and induced a weak, non-significant growth inhibitory effect on breast cancer multicellular tumor spheroids (3D). In addition, the cytotoxicity of NK cells against breast cancer cell lines was examined in 2D and 3D models, and beta-glucan significantly increased the cytotoxicity of NK cells against MCF-7 (in 2D). Yeast derived beta-(1,3)(1,6)-D-glucan could contribute to the treatment of cancer by enhancing NK cell immune response as well as contributing to inhibition of breast cancer cell growth.

中文翻译：

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酵母来源的 β-(1→3)(1→6)-D-葡聚糖对 2D 和 3D 培养物中自然杀伤 (NK) 细胞和乳腺癌细胞系的作用

β-(1,3)(1,6)-D-葡聚糖是一种复杂的多糖，存在于多种真菌、酵母、细菌、藻类、大麦和燕麦的细胞壁中，具有免疫调节、抗癌和抗病毒作用。在本研究中，我们在 2D 和 3D 模型中研究了源自酵母的 β-(1,3)(1,6)-D-葡聚糖对原代 NK 细胞和乳腺癌细胞系增殖的影响，以及对在 2D 和 3D 模型中原代 NK 细胞对乳腺癌细胞系的细胞毒性。在这项研究中，我们在2D和3D模型中研究了不同浓度的酵母来源的β-(1→3)(1→6)-D-葡聚糖对人类NK细胞和乳腺癌细胞系的增殖和细胞毒性的影响使用 XTT 细胞增殖测定和 CellTiter-Glo® 2.0 测定在 2D 和 3D 模型中确定人类 NK 细胞对乳腺癌细胞系的细胞毒性。我们发现，在不存在IL2的情况下，NK细胞与β-葡聚糖共孵育48小时显着增加了NK细胞的增殖，而在IL2存在的情况下，NK细胞与β-葡聚糖共孵育（70 U /ml) 增加了 NK 细胞的增殖，但不显着。此外，β-葡聚糖在2D模型中显着抑制乳腺癌细胞系的增殖，并对乳腺癌多细胞肿瘤球体（3D）产生微弱的、不显着的生长抑制作用。此外，在2D和3D模型中检查了NK细胞针对乳腺癌细胞系的细胞毒性，β-葡聚糖显着增加了NK细胞针对MCF-7的细胞毒性（在2D中）。酵母衍生的 β-(1,3)(1,6)-D-葡聚糖可以通过增强 NK 细胞免疫反应以及抑制乳腺癌细胞生长来治疗癌症。