Abstract

Slow wound healing in diabetic patients is a common complication of diabetes. Autologous conditioned serum (ACS) therapy is an emerging and safe biological therapy, and may accelerate the wound healing in diabetes. To investigate the effect of ACS in promoting wound healing in diabetic mice and its possible mechanism. Twenty-four six-week-old male C57BL/6 J mice were selected and divided into 5 groups, including control group (Ctrl), diabetic wound group (DW), ACS treatment group (DW+ACS) and STING pathway validation group (DW+ACS+DMXAA), with six mice in each group. Intervention was initiated after the back incision was performed, and wound healing was assessed on day 0, day 7, and day 14, and wound healing was assessed by hematoxylin and eosin (HE) staining of skin tissue on day 14. At the same time, the wound healing of the fibroblast markers collagen I and α-SMA was measured by immunohistochemistry and western blot. ACS treatment significantly accelerated the diabetic wound according to the wound area and HE staining results. Meanwhile, collagen I and α-SMA concentration evaluated by immunohistochemistry and western blot were remarkably elevated under the ACS interference. The STING signaling pathway was obviously activated in diabetic wound tissues. After the addition of DMXAA, an agonist of STING, the healing function of ACS was dramatically reversed. The application of ACS promotes wound healing in diabetic mice by enhancing fibroblasts. Meanwhile, the STING signaling pathway was inactivated by ACS interference. Hence, ACS can be used in the treatment of wound healing of Diabetes.

中文翻译：

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应用自体调理血清通过抑制 STING 通路激活对糖尿病小鼠伤口愈合的影响

摘要

糖尿病患者的伤口愈合缓慢是糖尿病的常见并发症。自体条件血清（ACS）疗法是一种新兴且安全的生物疗法，可以加速糖尿病的伤口愈合。探讨ACS对糖尿病小鼠伤口愈合的促进作用及其可能机制。选取六周龄雄性C57BL/6 J小鼠24只，分为5组，包括对照组（Ctrl）、糖尿病伤口组（DW）、ACS治疗组（DW+ACS）和STING通路验证组（DW+ACS）。 DW+ACS+DMXAA)，每组六只小鼠。背部切口后开始干预，第0天、第7天和第14天评估伤口愈合情况，第14天通过皮肤组织苏木精和伊红（HE）染色评估伤口愈合情况。 ，通过免疫组织化学和蛋白质印迹测量成纤维细胞标记物胶原蛋白 I 和 α-SMA 的伤口愈合情况。根据伤口面积和HE染色结果，ACS治疗显着加速了糖尿病伤口的愈合。同时，通过免疫组织化学和蛋白质印迹评估，在 ACS 干扰下，胶原蛋白 I 和 α-SMA 浓度显着升高。糖尿病伤口组织中STING信号通路明显激活。添加 STING 激动剂 DMXAA 后，ACS 的愈合功能显着逆转。ACS 的应用通过增强成纤维细胞来促进糖尿病小鼠的伤口愈合。同时，STING信号通路因ACS干扰而失活。因此，ACS可用于治疗糖尿病的伤口愈合。