Three-dimensional printing of cell constructs with high-cell density, shape fidelity, and heterogeneous cell populations is an important tool for investigating cell sociology in living tissues but remains challenging. Herein, we propose an artificial intercellular adhesion method using a photoresponsive chemical cue between a thiol-bearing polymer and a methacrylate-bearing cell membrane. This process provided cell fabrication containing 10⁸ cells/mL, embedded multiple cell populations in one structure, and enabled millimeter-sized scaleup. Our approach allows for the artificial cell construction of complex structures and is a promising bioprinting strategy for engineering tissues that are structurally and physiologically relevant.

中文翻译：

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通过光响应硫醇-烯反应直接制造糖工程细胞

具有高细胞密度、形状保真度和异质细胞群的细胞结构的三维打印是研究活组织中细胞社会学的重要工具，但仍然具有挑战性。在此，我们提出了一种利用含硫醇的聚合物和含甲基丙烯酸酯的细胞膜之间的光响应化学线索的人工细胞间粘附方法。这一过程提供了包含 10 ^{8 个}细胞/mL的细胞制造，将多个细胞群嵌入一个结构中，并实现了毫米级的放大。我们的方法允许复杂结构的人工细胞构建，并且是一种有前途的生物打印策略，用于工程结构和生理相关的组织。