Anion channelrhodopsin ACR1 is a powerful optogenetic tool to inhibit nerve activity. Its kinetic mechanism was interpreted in terms of the bacteriorhodopsin photocycle, and the L intermediate was assigned to the open channel state. Here, we report the results of the comparison between the time dependence of the channel currents and the time evolutions of the K-like and L-like spectral forms. Based on the results, we question the current view on ACR1 kinetics and the assignment of the L intermediate to the open channel state. We report evidence for a red-absorbing intermediate being responsible for channel opening.

中文翻译：

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阴离子通道视紫红质 GtACR1 中的开放通道状态是红色吸收中间体

阴离子通道视紫红质 ACR1 是一种抑制神经活动的强大光遗传学工具。其动力学机制根据细菌视紫红质光循环进行解释，并且L中间体被指定为开放通道状态。在这里，我们报告了通道电流的时间依赖性与 K 类和 L 类频谱形式的时间演化之间的比较结果。基于这些结果，我们质疑当前关于 ACR1 动力学的观点以及 L 中间体对开放通道状态的分配。我们报告了红色吸收中间体负责通道开放的证据。