The International Agency for Research on Cancer (IARC) classifies PFOA as a Class 1 carcinogen. Here, a new naked-eye PFOA immunochromographic strip was developed to recognize PFOA in domestic water and real human samples within 10 min based on a novel custom designed anti-PFOA monoclonal antibody (mAb) 2A3, which was firstly an immune rapid detection method for PFOA has been proposed. Using computer simulation techniques such as quantum computing to assist in designing the structural formula of PFOA semi antigen, which hapten was firstly proposed. The half maximal inhibitory concentration of PFOA monoclonal antibody (mAb) 2A3 was 2.4 μg/mL. Using mAb 2A3, we developed an immunochromatographic strip (ICS) for detecting PFOA in real samples. The developed method generated results in 10 min, with visual detection limits of 20, 20, and 200 μg/mL and limit of detection of 50, 200, and 500 μg/mL for water, blood and urine samples, respectively. The established ICS and indirect competitive enzyme-linked immunosorbent assay were used to analyze the actual samples, and the results were confirmed by LC-MS/MS. Our study findings showed that the ICS and ic-ELISA can quickly detect PFOA in actual samples.

中文翻译：

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视觉免疫层析条生物传感器现场快速检测生活用水和真实人体样本中的全氟辛酸

国际癌症研究机构 (IARC) 将 PFOA 列为 1 类致癌物。在此，基于新型定制设计的抗PFOA单克隆抗体（mAb）2A3，开发了一种新型裸眼PFOA免疫层析试纸条，可在10分钟内识别生活用水和真实人体样本中的PFOA，这是第一个针对PFOA的免疫快速检测方法。 PFOA 已被提出。利用量子计算等计算机模拟技术辅助设计PFOA半抗原的结构式，该半抗​​原是首次提出的。 PFOA 单克隆抗体 (mAb) 2A3 的半数最大抑制浓度为 2.4 μg/mL。使用 mAb 2A3，我们开发了一种免疫层析试纸条 (ICS)，用于检测实际样品中的 PFOA。所开发的方法可在 10 分钟内生成结果，水、血液和尿液样本的目视检测限分别为 20、20 和 200 μg/mL，检测限分别为 50、200 和 500 μg/mL。采用建立的ICS和间接竞争酶联免疫吸附法对实际样品进行分析，并通过LC-MS/MS对结果进行验证。我们的研究结果表明，ICS和ic-ELISA可以快速检测实际样品中的PFOA。