The endophytic fungus, Trichoderma sp. L2D2 was isolated from the medicinal plant Anaphalis contorta and has been assessed for extracellular enzyme production, plant growth promotion, antifungal, antibacterial, and antioxidant activities in vitro. The endophyte has been found to produce amylase, cellulose, and ammonia qualitatively. The antifungal activity was evaluated using Curvularia lunata, Fusarium oxysporum, Aspergillus niger, Aspergillus flavus, Sclerotium oryzae, Rhizoctonia solani, Alternaria brassicicola, Colletotrichum capsici, Ustilaginoidea virens, and Alternaria tenuissima by the dual culture method and showed strong antifungal activity with 100% inhibition against S. oryzae and C. capsici. For antibacterial activity, ethyl acetate extract of Trichoderma sp. L2D2 was tested against Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Salmonella typhi, Escherichia coli, and Shigella flexneri by the agar well diffusion method and the 96-well microplate method, and has shown the lowest MIC of 15.62 µg/ml against S. aureus and E. coli. The DPPH assay was used to examine the free radical scavenging activity of the crude extract of the endophytic fungus and showed good antioxidant activity with an IC₅₀ value of 85.94 µg/ml.

内生真菌，木霉属sp。L2D2 是从药用植物Anaphalis contorta中分离出来的，并已对其胞外酶产生、植物生长促进、抗真菌、抗菌和体外抗氧化活性进行了评估。已发现内生菌能够定性地产生淀粉酶、纤维素和氨。使用新月弯孢菌、尖孢镰刀菌、黑曲霉、黄曲霉、米菌核、立枯丝核菌、芸苔链格孢、辣椒炭疽菌、绿粉菌、细链格孢通过双重培养法评价其抗真菌活性，显示出很强的抗真菌活性，抑制率为100%对抗米链球菌和辣椒念珠菌。对于抗菌活性，木霉属的乙酸乙酯提取物。通过琼脂孔扩散法和 96 孔微孔板法对 L2D2 进行了针对枯草芽孢杆菌、金黄色葡萄球菌、粪肠球菌、伤寒沙门氏菌、大肠杆菌和福氏志贺氏菌的测试，结果显示，针对沙门氏菌的最低MIC为15.62 µg/ml。金黄色葡萄球菌和大肠杆菌。采用DPPH法检测内生真菌粗提物的自由基清除活性，显示出良好的抗氧化活性，IC ₅₀值为85.94 µg/ml。