Biomaterials containing citric acid as a building unit show potential for use as blood vessel and skin tissue substitutes. The success in commercializing implants containing a polymer matrix of poly(1,8-octanediol citrate) provides a rationale for exploring polycitrates based on other diols. Changing the aliphatic chain length of the diol allows functional design strategies to control the implant’s mechanical properties, degradation profile and surface energy. In the present work, poly(1,2-ethanediol citrate) was synthesized and used as an additive to polylactide in the electrospinning process. It was established that the content of polycitrate greatly influences the nonwovens’ properties: an equal mass ratio of polymers resulted in the best morphology. The obtained nonwovens were characterized by surface hydrophilicity, tensile strength, and thermal properties. L929 cell cultures were carried out on their surface. The materials were found to be non-cytotoxic and the degree of porosity was suitable for cell colonization. On the basis of the most important parameters for assessing the condition of cultured cells (cell density and viability, cell metabolic activity and lactate dehydrogenase activity), the potential of PLLA + PECit nonwovens for application in tissue engineering was established.

中文翻译：

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聚(1,2-乙二醇柠檬酸酯)/聚丙交酯非织造布在细胞培养中的应用探索

含有柠檬酸作为构建单元的生物材料显示出用作血管和皮肤组织替代品的潜力。含有聚（1,8-辛二醇柠檬酸酯）聚合物基质的植入物的商业化成功为探索基于其他二醇的聚柠檬酸酯提供了理论依据。改变二醇的脂肪链长度允许功能设计策略来控制植入物的机械性能、降解曲线和表面能。在目前的工作中，合成了聚（1,2-乙二醇柠檬酸酯）并在静电纺丝过程中用作聚丙交酯的添加剂。已确定聚柠檬酸酯的含量极大地影响非织造布的性能：等质量比的聚合物可产生最佳的形态。所得非织造布具有表面亲水性、拉伸强度和热性能等特征。L929细胞培养在其表面进行。发现该材料无细胞毒性，并且孔隙度适合细胞定植。根据评估培养细胞状况的最重要参数（细胞密度和活力、细胞代谢活性和乳酸脱氢酶活性），确定了 PLLA + PECit 非织造布在组织工程中应用的潜力。