Red blood cell (RBC) aggregation as well as their deformation significantly affects blood microrheology. These processes depend on various factors, one of which is concentration of the nitric oxide, one of the main signaling molecule in the bloodstream. The purpose of this study was to investigate the effect of nitric oxide on the microrheological properties of red blood cells (RBCs) in RBC samples of various media after the addition of nitric oxide donor sodium nitroprusside in vitro. Microrheological properties were measured using laser aggregometer and ektacytometer based on diffuse light scattering and diffraction of laser light on a suspension of RBCs, respectively. The study found that heparin-stabilized blood showed increased RBC aggregation and deformation with sodium nitroprusside concentrations of 100, and 200$$$\mu$M, while EDTA-stabilized blood showed slightly decreased aggregation and unchanged deformation. With washed RBCs in dextran solution, the addition of sodium nitroprusside (in the concentrations of 100, and 200$$$\mu$M) resulted in decreased aggregation and increased deformation. These findings aid in our understanding of nitric oxide’s effect on RBC microrheological properties.

红细胞（RBC）聚集及其变形显着影响血液微流变学。这些过程取决于多种因素，其中之一是一氧化氮的浓度，一氧化氮是血液中的主要信号分子之一。本研究的目的是在体外研究添加一氧化氮供体硝普钠后，一氧化氮对各种培养基红细胞样品中红细胞（RBC）微流变特性的影响。使用激光聚集计和激流计分别基于红细胞悬浮液上的漫射光散射和激光衍射来测量微流变特性。研究发现，硝普钠浓度为 100 和 200 时，肝素稳定的血液显示红细胞聚集和变形增加$$$\mu$M，而 EDTA 稳定的血液显示聚集略有减少，变形不变。将洗涤后的红细胞放入葡聚糖溶液中，加入硝普钠（浓度为 100 和 200$$$\mu$M)导致聚集减少和变形增加。这些发现有助于我们了解一氧化氮对红细胞微流变特性的影响。