Metallothioneins are a group of cysteine-rich proteins that play an important role in the homeostasis and detoxification of heavy metals. The objective of this research was to explore the significance of metallothionein in Trichoderma harzianum tolerance to zinc. At the inhibitory concentration of 1000 ppm, the fungus adsorbed 16.7 ± 0.4 mg/g of metal. The HPLC and SDS-PAGE electrophoresis data suggested that the fungus production of metallothionein was twice as high in the presence of zinc as in the control group. The examination of the genes; metallothionein expression activator (MEA) and Cu fist revealed that the MEA, with a C₂H₂ zinc finger domain, increased significantly in the presence of zinc. It was observed that in T. harzianum, the enhanced expression of the metallothionein gene was managed by the metallothionein activator under zinc overload conditions. According to our knowledge, this is the first report on the role of metallothionein in the resistance of T. harzianum to zinc.

金属硫蛋白是一组富含半胱氨酸的蛋白质，在重金属的体内平衡和解毒中发挥重要作用。本研究的目的是探讨金属硫蛋白在哈茨木霉对锌的耐受性中的意义。在1000 ppm的抑制浓度下，真菌吸附了16.7 ± 0.4 mg/g的金属。HPLC和SDS-PAGE电泳数据表明，在锌存在的情况下，真菌产生的金属硫蛋白是对照组的两倍。基因检查；_{金属硫蛋白表达激活剂（MEA）和Cu拳头显示，具有C 2} H ₂锌指结构域的MEA在锌存在下显着增加。据观察，在哈茨木霉中，金属硫蛋白基因的增强表达是由金属硫蛋白激活剂在锌超载条件下控制的。据我们了解，这是第一篇关于金属硫蛋白在哈茨木霉抗锌中作用的报道。