Background and objectiveThis study aimed to assess the protective ability of edaravone on testicular torsion–detorsion injury in rats.MethodsEighteen adult male Sprague–Dawley rats were randomly divided into three groups: Sham group (control, n = 6); testicular torsion/detorsion (T/D group, n = 6) and T/D+edaravone (T/D+E group, n = 6). The spermatic cords of rats of the T/D group and the T/D+E group were rotated 720° in a clockwise direction and maintained for 120 min in this torsion position. Around 90 min after the torsion, edaravone at a dose of 10 mg/kg dissolved in saline was administered IP to the T/D+E group. The testicle was counter‐rotated to its normal position to allow reperfusion for 4 h. Left testes of each animal were excised 240 min after beginning of reperfusion. Oxidative stress markers (TAS, TOS, SOD, and MDA) and apoptotic pathways (Caspase 3, Caspase 8, Caspase 9, Bcl‐2, and Bax,) were assessed by ELISA methods. Also, testicles were subjected to the histopathologic and ultrasound examinations.ResultsUltrasound imaging showed that edaravone reduced the surface area and increased vascularization in testicles with T/D (p < 0.0001, p < 0.05, respectively). Edaravone pretreatment markedly decreased the levels of MDA, TOS, Bcl‐2, Bax, Caspase 3, Caspase 8, and Caspase 9 (p < 0.0001). Also, it increased significantly TAS levels (p < 0.0001) and reduced insignificantly SOD activity. Histopathologic examinations demonstrated that edaravone significantly attenuated the histological damage caused by T/D in testicles.ConclusionTaken together, the findings indicate that pretreatment of edaravone has protective effect against testicular T/D injury.

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依达拉奉对大鼠睾丸扭转-扭转损伤的影响

背景与目的本研究旨在评价依达拉奉对大鼠睾丸扭转-扭转损伤的保护能力。方法将18只成年雄性Sprague-Dawley大鼠随机分为3组：Sham组（对照组，n= 6); 睾丸扭转/扭转（T/D 组，n= 6) 和 T/D+依达拉奉 (T/D+E 组，n= 6）。T/D组和T/D+E组大鼠精索顺时针方向旋转720°，并保持该扭转位置120 min。扭转后约90分钟，T/D+E组腹腔注射依达拉奉，剂量为10mg/kg，溶解在盐水中。将睾丸反向旋转至正常位置以允许再灌注 4 小时。再灌注开始后240分钟切除每只动物的左侧睾丸。通过 ELISA 方法评估氧化应激标记物（TAS、TOS、SOD 和 MDA）和凋亡途径（Caspase 3、Caspase 8、Caspase 9、Bcl-2 和 Bax）。同时对睾丸进行组织病理学和超声检查。结果超声成像显示依达拉奉可减少睾丸表面积并增加 T/D 的血管化（p< 0.0001，p< 0.05，分别）。依达拉奉预处理显着降低 MDA、TOS、Bcl-2、Bax、Caspase 3、Caspase 8 和 Caspase 9 的水平（p< 0.0001)。此外，它还显着提高了 TAS 水平（p< 0.0001) 并显着降低 SOD 活性。组织病理学检查表明，依达拉奉可显着减轻T/D引起的睾丸组织学损伤。结论综上所述，依达拉奉预处理对睾丸T/D损伤具有保护作用。