Abstract

The Cellulose Synthase gene (CS) superfamily and COBRA-like (COBL) gene family are essential for synthesizing cellulose and hemicellulose, which play a crucial role in cell wall biosynthesis and the hardening of plant tissues. Our study identified 126 ZbCS and 31 ZbCOBL genes from the Zanthoxylum bungeanum (Zb) genome. Phylogenetic analysis and conservative domain analysis unfolded that ZbCS and ZbCOBL genes were divided into seven and two subfamilies, respectively. Gene duplication data suggested that more than 75% of these genes had tandem and fragment duplications. Codon usage patterns analysis indicated that the ZbCS and ZbCOBL genes prefer ending with A/T base, with weak codon preference. Furthermore, seven key ZbCS and five key ZbCOBL genes were identified based on the content of cellulose and hemicellulose and the expression characteristics of ZbCS and ZbCOBL genes in various stages of stipule thorns. Altogether, these results improve the understanding of CS and COBL genes and provide valuable reference data for cultivating Zb with soft thorns.

中文翻译：

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花椒托叶刺纤维素合酶超家族和COBRA样基因家族基因组调查及表达分析

摘要

纤维素合酶基因（CS）超家族和COBRA样（COBL）基因家族是合成纤维素和半纤维素所必需的，在细胞壁生物合成和植物组织硬化中发挥着至关重要的作用。我们的研究从花椒( Zb ) 基因组中鉴定出 126 个ZbCS和 31 个ZbCOBL基因。系统发育分析和保守域分析表明ZbCS和ZbCOBL基因分别分为7个亚族和2个亚族。基因重复数据表明，超过 75% 的基因存在串联和片段重复。密码子使用模式分析表明，ZbCS和ZbCOBL基因更倾向于以A/T碱基结尾，密码子偏好较弱。进一步根据托叶刺中纤维素和半纤维素含量以及ZbCS和ZbCOBL基因在不同时期的表达特征，鉴定出7个关键ZbCS和5个关键ZbCOBL基因。总之，这些结果提高了对CS和COBL基因的认识，为软刺Zb的培育提供了有价值的参考数据。