There has been a growing interest in skin beauty and antimelanogenic products. Melanogenesis is the process of melanin synthesis whereby melanocytes are activated by UV light or hormone stimulation to produce melanin. Melanogenesis is mediated by several enzymes, such as tyrosinase (TYR), microphthalmia-associated transcription factor (MITF), tyrosinase-related protein-1 (TRP-1), and TRP-2. In this study, we investigated the effect of Tuber himalayense extract on melanin synthesis in α-melanocyte-stimulating hormone (α-MSH)-treated B16F10 melanoma cells. We confirmed that T. himalayense extract was not toxic to α-MSH-treated B16F10 melanoma cells and exhibited a significant inhibitory effect on melanin synthesis at concentrations of 25, 50, and 100 μg/ml. Additionally, the T. himalayense extract inhibited melanin, TRP-1, TRP-2, tyrosinase, and MITF, which are enzymes involved in melanin synthesis, in a concentration-dependent manner. Furthermore, T. himalayense extract inhibited the mitogen-activated protein kinase (MAPK) pathways, such as extracellular signal-regulated kinase-1/2 (ERK), c-Jun N-terminal kinase (JNK), and p38. Therefore, we hypothesized that various components of T. himalayense extract affect multiple factors involved in melanogenesis in B16F10 cells. Our results indicate that T. himalayense extract could potentially be used as a new material for preparing whitening cosmetics.

中文翻译：

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韩国仁川分离的喜马拉雅块茎的黑色素抑制作用。

人们对皮肤美容和抗黑色素生成产品越来越感兴趣。黑色素生成是黑色素合成的过程，其中黑素细胞被紫外线或激素刺激激活以产生黑色素。黑色素生成由多种酶介导，例如酪氨酸酶 (TYR)、小眼相关转录因子 (MITF)、酪氨酸酶相关蛋白 1 (TRP-1) 和 TRP-2。在这项研究中，我们研究了喜马拉雅块茎提取物对 α-黑素细胞刺激激素 (α-MSH) 处理的 B16F10 黑色素瘤细胞中黑色素合成的影响。我们证实喜马拉雅木霉e 提取物对 α-MSH 处理的 B16F10 黑色素瘤细胞没有毒性，并且在浓度为 25、50 和 100 μg/ml 时对黑色素合成表现出显着的抑制作用。此外，喜马拉雅木提取物以浓度依赖性方式抑制黑色素、TRP-1、TRP-2、酪氨酸酶和 MITF（这些酶是参与黑色素合成的酶）。此外，T. Himalayense提取物抑制丝裂原激活蛋白激酶 (MAPK) 通路，例如细胞外信号调节激酶-1/2 (ERK)、c-Jun N 末端激酶 (JNK) 和 p38。因此，我们假设喜马拉雅木提取物的多种成分影响 B16F10 细胞黑素生成中涉及的多种因素。我们的结果表明，喜马拉雅木提取物有可能用作制备美白化妆品的新材料。