Hydrogen peroxide (HO) is a reactive oxygen species (ROS) that can be used as a marker for the occurrence of oxidative stress in the organism. Lysosomes serve as intracellular digestive sites, and when the concentration of HO in them is abnormal, lysosomal function is often impaired, leading to the development of diseases. Hydrogen sulfide (HS) acts as a gaseous signaling molecule that scavenges HO from cells and tissues, thereby maintaining the redox environment of the body. However, most of the reported hydrogen peroxide fluorescent probes so far can only detect HO, but cannot maintain the intracellular redox environment. In this paper, an HO fluorescent probe with lysosomal targeting properties was designed and synthesized by combining the HO recognition site with a naphthylamine fluorophore via a thiocarbamate moiety. The probe has the advantages of large Stokes shift (110 nm), high sensitivity and good HS release capability. The probe can be used to detect HO in cells, zebrafish and plant roots. In addition, detects changes in the concentration of HO in plant roots when is stressed by cadmium ion (Cd). And through its ability to release HS, it can help to remove excess HO and maintain the redox environment in cells, zebrafish and plant roots. The present work provides new ideas for the detection and assisted removal of HO, which contributes to the in-depth study of the cellular microenvironment in organisms.

中文翻译：

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溶酶体中检测H2O2和传递H2S的荧光探针及其在维持氧化还原环境中的应用

过氧化氢（H2O）是一种活性氧（ROS），可用作生物体中氧化应激发生的标志物。溶酶体作为细胞内的消化场所，当其中H2O浓度异常时，溶酶体功能往往会受损，导致疾病的发生。硫化氢 (HS) 作为一种气体信号分子，从细胞和组织中清除 H2O，从而维持体内的氧化还原环境。然而，迄今为止报道的大多数过氧化氢荧光探针只能检测H2O，而不能维持细胞内的氧化还原环境。本文通过硫代氨基甲酸酯部分将 H2O2 识别位点与萘胺荧光团结合，设计并合成了具有溶酶体靶向特性的 H2O2 荧光探针。该探针具有斯托克斯位移大(110 nm)、灵敏度高、H2S释放能力好等优点。该探针可用于检测细胞、斑马鱼和植物根中的 H2O。此外，还可检测镉离子 (Cd) 胁迫时植物根部 H2O 浓度的变化。通过其释放 H2S 的能力，它可以帮助去除多余的 H2O，维持细胞、斑马鱼和植物根部的氧化还原环境。本工作为H2O的检测和辅助去除提供了新思路，有助于深入研究生物体细胞微环境。