This work aims to develop a method for analyzing the dynamic flow of single DNA molecules through nanochannels in fluidic chip which work passively. For this purpose, a two-laser system was used, which is coupled into a fluorescence microscope and can detect labeled DNA molecules using a single photon counter for signal read-out in real-time. The two laser spots are focused at different points of the nanochannel, and can be used to obtain the speed and molecular extension of the DNA. For example, a velocity of 1.82 μm/ms and a spatial extension of 0.9 μm were determined for pUC18/19 DNA (2686 bp) in a nanochannel with a width and depth of 200 nm × 100 nm and a length of 10 μm. © 2024 Institute of Electrical Engineer of Japan and Wiley Periodicals LLC.

中文翻译：

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被动纳米流体装置中 DNA 分子的流动行为表征†

这项工作旨在开发一种方法来分析单个 DNA 分子通过被动工作的流体芯片中的纳米通道的动态流动。为此，使用了双激光系统，该系统与荧光显微镜耦合，可以使用单光子计数器实时读出信号来检测标记的 DNA 分子。两个激光点聚焦在纳米通道的不同点，可用于获得DNA的速度和分子延伸。例如，在宽度和深度为200 nm×100 nm、长度为10 μm的纳米通道中，确定pUC18/19 DNA（2686 bp）的速度为1.82 μm/ms，空间延伸为0.9 μm。 © 2024 日本电气工程师协会和 Wiley periodicals LLC。