Despite recent advances in the diagnosis and treatment of oral cancer, relative survival rates have not changed significantly. Moringa oleifera (M. oleifera) Lam. is one such plant with its anticancer properties being proved in its leaves, stem, flowers but no studies are yet reported proving the anticancer property of its seed oil on oral cancer. This study aimed to evaluate the anti-proliferative and cytotoxic effect of M. oleifera seed oil against two Oral squamous cell carcinoma cell lines CAL27 and SCC15 using MTT assay. 2D GC-TOF Mass spectrometry revealed a total of 199 compounds, among which the majority were alkanes (68.2016%) and fatty acid esters (11.1399%). The MTT assay report showed good dose-dependent activity. A significant reduction in cell viability within 24 h with IC50 value of 17.78 µg/mL and 24.28 µg/mL for all treatment groups was observed for both the cell lines CAL27 and SCC15. MTT assay showed a significant decrease in cell viability with an increase in the oil dose, thereby revealing the cytotoxic and anti-proliferative activity of M. oleifera seed oil on oral cancer cell lines namely CAL27and SCC15. The results of this study indicate that M. oleifera seed oil can be used as a potent anti-cancer agent in the treatment of Oral cancer.

中文翻译：

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辣木的抗癌活性。种子油治疗口腔癌

尽管口腔癌的诊断和治疗最近取得了进展，但相对生存率并没有显着变化。辣木 (M. oleifera) Lam。就是这样一种植物，其叶、茎、花的抗癌特性已得到证实，但尚未有研究报道证明其种子油对口腔癌具有抗癌特性。本研究旨在利用MTT法评估油橄榄籽油对两种口腔鳞状细胞癌细胞系CAL27和SCC15的抗增殖和细胞毒性作用。2D GC-TOF 质谱共检测出 199 种化合物，其中大部分为烷烃（68.2016%）和脂肪酸酯（11.1399%）。MTT测定报告显示出良好的剂量依赖性活性。对于 CAL27 和 SCC15 细胞系，所有处理组的细胞活力在 24 小时内显着降低，IC50 值为 17.78 µg/mL 和 24.28 µg/mL。MTT 测定显示，随着油剂量的增加，细胞活力显着降低，从而揭示了油茶籽油对口腔癌细胞系 CAL27 和 SCC15 的细胞毒性和抗增殖活性。这项研究的结果表明，油橄榄籽油可用作治疗口腔癌的有效抗癌剂。