Purpose: Bladder cancer (BC) is one of the top 10 common tumors in the world. It has been reported that microbiota can colonize tissues and play important roles in tumorigenesis and progression. However, the current understanding of microorganisms in the BC tissue microenvironment remains unclear./nMethods: In this study, we integrated the RNA-seq data of 479 BC tissue samples from seven datasets combined with a range of bioinformatics tools to explore the landscape of microbiome in the BC tissue microenvironment./nResults: The pan-microbiome was estimated to surpass 1,400 genera. A total of seven core microbiota (Bacillus, Corynebacterium, Cutibacterium, Escherichia, Halomonas, Pasteurella, and Streptomyces) were identified. Among them, Bacillus was widely distributed in all datasets with a high relative abundance (10.11% of all samples on average). Moreover, some biological factors, including tissue source and tumor grade, were found significant effects on the microbial composition of the bladder tissue. Pseudomonas, Porphyrobacter, and Acinetobacter were enriched in tumor tissues, while Mycolicibacterium and Streptomyces were enriched in patients who showed durable response to BCG therapy. In addition, we established microbial co-occurrence networks and found that the BCG therapy may attenuate the microbiological interactions./nConclusions: This study clearly provided a microbial landscape of the BC tissue microenvironment, which was important for exploring the interactions between microorganisms and BC tissues. The identified specific taxa might be potential biomarkers for BC.

中文翻译：

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利用 RNA 测序数据揭示膀胱癌组织微环境的微生物组成

目的：膀胱癌（BC）是世界十大常见肿瘤之一。据报道，微生物群可以在组织中定殖，并在肿瘤的发生和进展中发挥重要作用。然而，目前对 BC 组织微环境中微生物的了解仍不清楚。/n方法：在这项研究中，我们整合了来自 7 个数据集的 479 个 BC 组织样本的 RNA-seq 数据，并结合一系列生物信息学工具来探索BC 组织微环境中的微生物组。/n结果：泛微生物组估计超过 1,400 个属。总共鉴定了七种核心微生物群（芽孢杆菌属、棒状杆菌属、皮肤杆菌属、埃希氏菌属、盐单胞菌属、巴斯德氏菌属和链霉菌属）。其中，芽孢杆菌在所有数据集中分布广泛，相对丰度较高（平均占所有样本的10.11%）。此外，一些生物因素，包括组织来源和肿瘤分级，被发现对膀胱组织的微生物组成有显着影响。假单胞菌属、卟啉杆菌属和不动杆菌属在肿瘤组织中富集，而分枝杆菌属和链霉菌属在对卡介苗治疗表现出持久反应的患者中富集。此外，我们建立了微生物共现网络，发现卡介苗治疗可能会减弱微生物相互作用。/n结论：这项研究清楚地提供了BC组织微环境的微生物景观，这对于探索微生物与BC之间的相互作用具有重要意义组织。确定的特定分类单元可能是 BC 的潜在生物标志物。