Pathogenic diseases that trigger food safety remain a noteworthy concern due to substantial public health, economic, and social burdens worldwide. It is vital for developing an integrated diagnosis and treatment strategy for bacteria, which could achieve quick detection of pathogenic bacteria and the inhibition of multidrug-resistant bacteria. Herein, we reported an organic molecule (M-3) possessed strong light capture capacity, emerging a low energy gap and ΔE_ST. Subsequently, M-3 was integrated into a nanostructured system (BTBNPs) with excellent ROS generation, light absorption capability, and photothermal performance. Reactive oxygen species (ROS) generated by BTBNPs were mainly free radicals from a type I mechanism, and the high photothermal conversion efficiency of BTBNPs was 41.26%. Benefiting from these advantages of BTBNPs, BTBNPs could achieve a ∼99% antibacterial effect for Escherichia coli O157:H7 with 20 μM dosage and 5 min of irradiation. Furthermore, the limit of detection (LoD) of the proposed BTBNPs-LFIA (colorimetric and photothermal modalities) for detecting E. coli O157:H7 was 4105 and 419 CFU mL^–1, respectively. Overall, this work is expected to provide a new and sophisticated perspective for integrated diagnosis and treatment systems regarding pathogenic bacteria.

中文翻译：

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高性能多功能纳米粒子研发助力大肠杆菌O157:H7一体化诊疗

由于全球巨大的公共卫生、经济和社会负担，引发食品安全的致病性疾病仍然是一个值得注意的问题。制定细菌综合诊断和治疗策略，实现病原菌的快速检测和多重耐药菌的抑制至关重要。在此，我们报道了一种有机分子（M-3）具有很强的光捕获能力，具有低能隙和Δ E _ST。随后，M-3被集成到纳米结构系统（BTBNPs）中，具有优异的ROS生成、光吸收能力和光热性能。 BTBNPs产生的活性氧(ROS)主要是I型机制的自由基，BTBNPs的光热转换效率高达41.26%。受益于 BTBNPs 的这些优点，BTBNPs在 20 μM 剂量和照射 5 分钟的情况下对大肠杆菌O157:H7可以达到~99% 的抗菌效果。此外，所提出的用于检测大肠杆菌O157:H7的 BTBNPs-LFIA（比色和光热模式）的检测限 (LoD)分别为 4105 和 419 CFU mL ^–1。总的来说，这项工作有望为病原菌的综合诊断和治疗系统提供新的、复杂的视角。